An improved plating assay for determination of phage titer

Liu Yang; Chao Li; Shao-Qin Zhai; Hua Zheng; Li-Zhi Fu; Da-Jun Li

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An improved plating assay for determination of phage titer

机译：用于测定噬菌体滴度的改进的平板检测

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Phage is a virus that is parasitic on bacteria. It is very important to determine the titer of test sample in the study of phage. In this study, an improved plating assay was developed for detection of the number of recombinant phage Cap-T7 present in a test solution at a certain dilution point by counting the plaque forming units. The data demonstrated that the improved plating assay is fast, useful, and convenient for the determination of the phage titer in a sample.

机译：噬菌体是一种寄生在细菌上的病毒。在噬菌体研究中确定测试样品的滴度非常重要。在这项研究中，开发了一种改进的平板测定法，用于通过计算噬菌斑形成单位来检测在特定稀释点存在于测试溶液中的重组噬菌体Cap-T7的数量。数据表明，改进的平板测定法可快速，有用和方便地测定样品中的噬菌体滴度。

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来源
《African Journal of Biotechnology》 |2016年第23期|共5页
作者
Liu Yang; Chao Li; Shao-Qin Zhai; Hua Zheng; Li-Zhi Fu; Da-Jun Li;
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中图分类生物工程学（生物技术）;
关键词
Phage Cap-T7detection methodplaque forming units;

机译：噬菌体Cap-T7检测方法噬菌斑形成单位;

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5. Development of a luciferase reporter phage assay for detection of Mycobacterium avium subsp. paratuberculosis [D] . McCusker, Susan Elizabeth 2005

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6. Correction: Isolation of Phages for Phage Therapy: A Comparison of Spot Tests and Efficiency of Plating Analyses for Determination of Host Range and Efficacy [O] . Mohammadali Khan Mirzaei, Anders S. Nilsson -1

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An improved plating assay for determination of phage titer

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