首页> 外文期刊>African Journal of Biotechnology >Effects of rare earth elements on callus growth, soluble protein content, peroxidase activity and shoot differentiation of Echinacea angustifolia cultures in vitro
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Effects of rare earth elements on callus growth, soluble protein content, peroxidase activity and shoot differentiation of Echinacea angustifolia cultures in vitro

机译:稀土元素对紫锥菊培养物愈伤组织生长,可溶性蛋白含量,过氧化物酶活性和芽分化的影响

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The effects of?lanthanum nitrate (La3+) and cerium nitrate (Ce4+) on?Echinaceaangustifolia?callus growth and subculture were studied by the measurement of callus fresh weight, dry weight and time-course curve. The effects of La3+?on soluble protein?content, peroxidase activity and shoot differentiation of callus were also investigated. Low concentrations of La3+?and Ce4+?(0.01, 0.1 and 1 mg/l) showed enhancing action on callus growth while the suppress effect was found at high concentration treatments (10 and 100 mg/l). The result from time-course experiment indicated that La3+?or Ce4+?showed promotion on callus growth after 15 days cultured and Ce4+?displayed a better effect than La3+. Different proportion mixture of La3+?and Ce4+?at 1 mg/l in total showed stimulating action on callus growth and the optimum proportion was the mixture of 40% La3+?and 60% Ce4+. Inhibitory effects were observed when the calli derived from the treatments with La3+?and Ce4+?were subcultured for the?first generation. The dose-dependent effects of La3+?on soluble protein content, peroxidase activity and shoot differentiation were also noticed. The optimum concentration for callus growth and shoot differentiation was 0.1 mg/l and the green, loose calli with low percentage of brown callus were obtained at this concentration.
机译:通过测量愈伤组织的鲜重,干重和时程曲线,研究了硝酸镧(La3 +)和硝酸铈(Ce4 +)对紫锥菊(Echinaceaangustifolia)愈伤组织生长和继代培养的影响。还研究了La3 +对可溶性蛋白质含量,过氧化物酶活性和愈伤组织芽分化的影响。低浓度的La3 +α和Ce4 +α(0.01、0.1和1 mg / l)表现出对愈伤组织生长的增强作用,而高浓度处理(10和100 mg / l)则具有抑制作用。时程实验结果表明,培养15天后La3 +或Ce4 +对愈伤组织的生长有促进作用,而Ce4 +比La3 +具有更好的生长效果。总量为1 mg / l的La3 +?和Ce4 +?的不同比例混合物显示出对愈伤组织生长的刺激作用,最佳比例为40%La3 +?和60%Ce4 +的混合物。当将第一代La3 +和Ce4 +处理的愈伤组织继代培养时,观察到了抑制作用。还观察到La3 +?对可溶性蛋白含量,过氧化物酶活性和芽分化的剂量依赖性作用。愈伤组织生长和芽分化的最佳浓度为0.1 mg / l,在该浓度下获得了绿色,稀疏的愈伤组织,其棕色愈伤组织的百分含量低。

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