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首页> 外文期刊>African Journal of Biotechnology >Purification and identification of a phytase from fruity bodies of the winter mushroom, Flammulina velutipes
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Purification and identification of a phytase from fruity bodies of the winter mushroom, Flammulina velutipes

机译：冬菇金针菇果体内植酸酶的纯化与鉴定

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摘要

A phytase, with a molecular mass of 14.8 kDa as determined by gel filtration and sodium dodecyl?sulfate-polyacrylamide gel electrophoresis was isolated from fresh fruiting bodies of the mushroom?Flammulina velutipes. The chromatographic procedure used for isolation of the phytase included ion exchange chromatography on DEAE-cellulose, CM-cellulose, Q-Sepharose, affinity chromatography on Affi-gel blue gel and fast protein liquid chromatography-gel filtration on Superdex 75. The enzyme was adsorbed on DEAE-cellulose, unadsorbed on CM-cellulose and Affi-gel blue gel, and adsorbed on Q-Sepharose. It presented an N-terminal sequence different from those of reported fungal phytases. It exhibited maximal activity at about 45°C and it did not manifest drastic fluctuations in activity over the pH of 3 to?9. The phytase presented activity towards a variety of phosphorylated compounds with the following ranking of activity: ATP > fructose-6-phosphate ≈ glucose-6-phosphate > AMP > ADP> β-glycerophosphate > sodium phytate. It did not present antifungal activity.
机译：从蘑菇？金针菇的新鲜子实体中分离出了一种植酸酶，其分子量为14.8 kDa（通过凝胶过滤和十二烷基硫酸钠-聚丙烯酰胺凝胶电泳）。用于分离肌醇六磷酸酶的色谱方法包括：DEAE-纤维素，CM-纤维素，Q-Sepharose上的离子交换色谱，Affi-gel蓝凝胶上的亲和色谱和Superdex 75上的快速蛋白质液相色谱-凝胶过滤。酶被吸附在DEAE-纤维素上吸附，在CM-纤维素和Affi-gel蓝色凝胶上未吸附，在Q-Sepharose上吸附。它呈现出不同于已报道的真菌植酸酶的N端序列。它在约45℃下表现出最大的活性，并且在3至〜9的pH范围内没有表现出剧烈的活性波动。肌醇六磷酸酶对多种磷酸化的化合物具有活性，其活性如下：ATP> 6磷酸果糖≈6磷酸葡萄糖> AMP> ADP>β-甘油磷酸>植酸钠。它没有表现出抗真菌活性。

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