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首页> 外文期刊>African Journal of Biotechnology >Over-expressing Salicornia europaea (SeNHX1) gene in tobacco improves tolerance to salt
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Over-expressing Salicornia europaea (SeNHX1) gene in tobacco improves tolerance to salt

机译：烟草中过表达Salicornia europaea（SeNHX1）基因可提高对盐的耐受性

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摘要

Vacuolar Na+/H+?antiporters provide an efficient mechanism to avert the deleterious effects of Na+?by its compartmentalization into vacuoles. In this study,?SeNHX1, a vacuolar Na+/H+?antiporter from?Salicornia europaea.L, was introduced into tobacco to investigate its response to salt tolerance, and to study how over-expression of this gene can affect other physiological responses. An increase of salt tolerance in transgenics was observed both?in vitro?and in pot culture. When exposed to either?138 mM or 172 mM?NaCl?in vitro, the transgenic?apical meristem and stem segments with buds?exhibited stronger salt-tolerance than their wild-type counterparts. In pot soil?with 10.2 mg g-1?DW Na+?stress,?transgenic plants over-expressing?SeNHX1 accumulated 1.2 mg g-1?FW Na+?greater than wild type in old leaves. Meanwhile, no difference was observed in young leaves. Dry weight and height of transgenic plants were reduced less compared to that of wild type. Moreover, under high salt stress, the malondialdehyde (MDA) content in transgenic plants was significantly lower, but proline content and activity of antioxidant enzymes were obviously higher?than that of?wild-types. These results confirm and support the potential application of?SeNHX1-transgenic plants for being cultivated in saline soil.
机译：液泡Na + / H +-反向转运蛋白提供了一种有效的机制，通过将其分隔成液泡来避免Na +-的有害作用。在这项研究中，将Señcorniaeuropaea.L的液泡型Na + / H +β反向转运蛋白SeNHX1引入烟草中，以研究其对耐盐性的反应，并研究该基因的过表达如何影响其他生理反应。在体外和盆栽培养中均观察到转基因植物的耐盐性增加。当在体外暴露于138 mM或172 mM NaCl中时，转基因的带有芽的茎尖分生组织和茎节段比野生型对应物表现出更强的耐盐性。在10.2 mg g-1？DW Na +胁迫下的盆栽土壤中，过表达？SeNHX1的转基因植物在老叶中比野生型积累了1.2 mg g-1？FW Na +。同时，在嫩叶中未观察到差异。与野生型相比，转基因植物的干重和高度降低得较少。此外，在高盐胁迫下，转基因植物的丙二醛（MDA）含量明显降低，但脯氨酸含量和抗氧化酶活性明显高于野生型。这些结果证实并支持了转基因SeSeNX1转基因植物在盐渍土壤中的潜在应用。

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