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A molecular phylogeny of selected species of Genus Prunus L. (Rosaceae) from Pakistan using the TRN-L TRN-F spacer DNA

机译:使用TRN-L TRN-F间隔子DNA从巴基斯坦选择的樱属(蔷薇科)种的分子系统发育

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The genus?Prunus?L. is an important plant for fruit production and it includes plums, apricots, cherries, almonds and peaches that are sources of food for the local people. The family?Rosaceae?is not yet published in Flora of Pakistan and there is a lot of taxonomic work that is yet to be done for the proper classification and placement of different genera under different sub-families.?Prunus?is found in almost all the four provinces of Pakistan including Punjab, Khyber Pakhtunkhwa (former NWFP), Sindh and Baluchistan which includes Azad Kashmir region. In the present study, the genus?Prunus?was studied in detail to find out the phylogenetic relationship among the 12 species of?Prunus?selected from different regions of Pakistan and GenBank using the maximum parsimony analysis of sequence polymorphism in chloroplast TRN-L and TRN-F spacer DNA. The results for the TRN-L and TRN-F primers confirm the work done by early phylogenetists including Potter and Bortiri with additions to new species from Pakistan including?Prunus dulcis?(Mill.) D.A. Webb. (Syn.?Prunus amygdalus) and?Prunus cornuta?(Wall. ex. Royle) Steudel. which are indigenous to Pakistan.?Key Words:?Prunus, chloroplast, TRN-L, TRN-F, Pakistan.
机译:属?李?是水果生产的重要植物,其中包括李子,杏子,樱桃,杏仁和桃子,这是当地人的食物来源。蔷薇科(Rosaceae)家族尚未在巴基斯坦的植物群中发表,为将不同属正确分类和放置在不同亚科之下,还有许多分类工作要做。在几乎所有的亚科中都发现了李。巴基斯坦的四个省包括旁遮普省,开伯尔·普赫图赫瓦省(前NWFP),信德省和Bal路支斯坦省,其中包括Azad克什米尔地区。在本研究中,通过对叶绿体TRN-L和SNP的序列多态性的最大简约分析,对Prunus属进行了详细的研究,以找出从巴基斯坦和GenBank不同地区选择的12种Prunus之间的系统发生关系。 TRN-F间隔DNA。 TRN-L和TRN-F引物的结果证实了早期系统发育学家(包括Potter和Bortiri)所做的工作,此外还添加了来自巴基斯坦(Prunus dulcis?)的新物种。韦伯。 (Syn.?Prunus amygdalus)和?Prunus cornuta?(Wall。ex。Royle)Steudel。关键词:李,叶绿体,TRN-L,TRN-F,巴基斯坦。

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