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首页> 外文期刊>African Journal of Biotechnology >Antimicrobial activity analysis of extracts of Acacia modesta, Artimisia absinthium, Nigella sativa and Saussurea lappa against Gram positive and Gram negative microorganisms
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Antimicrobial activity analysis of extracts of Acacia modesta, Artimisia absinthium, Nigella sativa and Saussurea lappa against Gram positive and Gram negative microorganisms

机译：相思树，苦艾，苦艾草和雪莲提取物对革兰氏阳性和革兰氏阴性微生物的抗菌活性分析

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摘要

Antimicrobial activity of extracts of certain herbs including?Acacia modesta?(leaf and stem),?Artimisia absinthium?(leaf and stem), Nigella sativa?(seeds)?and Saussurea lappa?(root)?was evaluated against three Gram positive and two Gram negative microorganisms. The Gram positive organisms included?Bacillus subtalis (ATCC 6633), Entereococcus faecalis (ATCC 14506)?and?Staphylococcus aureus?(ATCC 6538) and the Gram negative organisms included?Pseudomonas aeruginosa (ATCC 27853)?and?Salmonella typhi (ATCC 14028).?Methanolic, hot water and cold water extracts of these plants were taken for antibacterial assay through Discs agar diffusion technique using commercial filter paper discs applied on inoculated Muellar Hinton agar plates. The objective of this?study?was to explore the curative powers of these herbs that exist in nature as a tool to counter disease causing agents. The maximum zone of inhibition of 18 mm of methanolic extract of?N. sativawas observed against?B. subtalis?and?S. aureus.?Similarly, the maximum zone of inhibition of 18 mm of cold water extract of?N. sativa?against?S. aureus?and methanolic extract of?Saussurea lappa?against?S. aureus?and?Pseudomonas aeruginosa?was also observed.?The antibacterial action was compared with the effect of ceftriaxone, ceftriaxone sodium, cefuroxine, ciprofloxacin, gentamycine, levofloxacin, metronidazole and tranexamic acid that were used as standered drugs. Based on the results obtained in this?study, it may be concluded that plant extracts of?A. modesta, A. absinthium, N. sativa and Saussurea lappa?have a stronger?and broader spectrum of antimicrobial activity against a number of food borne bacteria.
机译：评估了某些草药提取物的抗菌活性，包括“相思树”（叶和茎），“苦艾蒿”（叶和茎），“黑草”（种子）和“雪莲”（根）对三种革兰氏阳性和两种革兰氏阴性微生物。革兰氏阳性菌包括：枯草芽孢杆菌（ATCC 6633），粪肠球菌（ATCC 14506）和金黄色葡萄球菌（ATCC 6538），革兰氏阴性菌包括铜绿假单胞菌（ATCC 27853）和伤寒沙门氏菌（ATCC 14028）。这些植物的甲醇，热水和冷水提取物通过Discs琼脂扩散技术，采用商业的滤纸圆盘应用到接种的Muellar Hinton琼脂平板上，通过Discs琼脂扩散技术进行抗菌测定。这项研究的目的是探索天然存在的这些草药作为对抗致病因子的工具的疗效。 18 N甲醇提取物的最大抑制区。观察到苜蓿对？B。下tal骨和S.同样，最大抑制区为18 mm的NN冷水提取物。苜蓿？金黄色葡萄球菌和雪莲的甲醇提取物对付S。还观察到了金黄色和铜绿假单胞菌的抗菌作用。并与用作标准药物的头孢曲松，头孢曲松钠，头孢呋辛，环丙沙星，庆大霉素，左氧氟沙星，甲硝唑和氨甲环酸的作用进行了比较。根据这项研究获得的结果，可以得出结论：？A的植物提取物。 modesta，A。absinthium，N。sativa和Saussurea lappa对多种食源性细菌具有更强，更广的抗菌活性。

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