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首页> 外文期刊>African Journal of Biotechnology >Expression of a SK2-type dehydrin gene from Populus euphratica in a Populus tremula Populus alba hybrid increased drought tolerance
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Expression of a SK2-type dehydrin gene from Populus euphratica in a Populus tremula Populus alba hybrid increased drought tolerance

机译：胡杨中的胡杨SK2型脱水蛋白基因的表达提高了白杨的耐旱性

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摘要

A dehydrin gene, isolated from cDNA library established from the root of?Populus euphratica, was determined from sequence analysis to be an SK2-type dehydrin (Pedhn). To investigate the function of?Pedhn, it was expressed via the CaMV 35S promoter in transgenic?Populus tremula × Populus alba. The?Pedhn?transgenic lines demonstrated higher water retention capacities in excised leaves of transgenic lines than that of wild type, and the rate of water loss in the leaves of transgenic lines were slower than that of wild type controls under drought stress. Higher water retention capacity and reduced water loss suggest that the transgenic lines would exhibit enhanced drought tolerance. Consistently, the seedlings of transgenic lines did have significantly enhanced drought tolerance when compared with that of the wild type controls under drought challenge, which indicated that expression ofPedhn?could be used to genetically modify poplar to improve drought tolerance.
机译：从序列分析从胡杨根部建立的cDNA文库中分离出的脱水蛋白基因经序列分析确定为SK2型脱水蛋白（Pedhn）。为了研究ΔPedhn的功能，它通过CaMV 35S启动子在转基因ΔPemulustremula×Populus alba中表达。在干旱胁迫下，“ Pedhn”转基因品系在转基因品系的切下的叶片中比野生型具有更高的保水能力，并且转基因品系的叶片中水分流失速率比野生型对照慢。较高的保水能力和减少的水分流失表明转基因品系将表现出增强的耐旱性。一致地，与干旱胁迫下的野生型对照相比，转基因品系的幼苗确实具有显着增强的耐旱性，这表明Pedhn？的表达可用于遗传修饰杨树以提高耐旱性。

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