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Effects of plant growth regulators on in vitro propagation of Cymbidium faberi Rolfe

机译：植物生长调节剂对大花ym兰体外繁殖的影响

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Cymbidium?faberi?Rolfe is one of the oldest cultivated orchids in China, which is often sold as a precious potted specimen because of its beautiful and fragrant flowers in eastern Asia. However, the propagation of?C.?faberi?is greatly restricted by the low seed germination rate due to immature?embryo?and no endosperm under natural conditions. In this study, the effects of plant growth regulators on?in vitropropagation of?C.?faberi?were investigated to establish?in vitro?propagation protocol. The results demonstrated that seeds began to form into white spots on 2.0 mg/L N6-benzyladenine (BA) + 1.5 mg/L α-naphthaleneacetic acid (NAA) and the highest number was on 2.0 mg/L BA + 2.0 mg/L NAA. And 1/2 Murashige and Skoog (MS) medium + 0.5 mg/L NAA was the optimal medium for protocorm-like bodies (PLB) turning green. In the subculture of PLB, 1/2 MS supplemented with NAA was more effective, its optimal concentration for growth and multiplication of PLB was 2.0 and 1.0 mg/L, respectively. In adventitious shoots induction, the optimal medium was 1/2 MS + 1.0 mg/L thidiazuron (TDZ) + 0.5 mg/L NAA whose induction rate was up to 56.8%. Additionally, 1/2 MS supplemented with 0.5 mg/L indole-3-butyric acid (IBA) was the best culture medium for rooting.?These results may provide available technique for cultivar breeding and?large-scale propagation?of?C. faberi.
机译：大花ym兰（Cymbidium？faberi？Rolfe）是中国最古老的栽培兰花之一，由于其东亚地区美丽而芬芳的花朵，通常作为珍贵的盆栽标本出售。但是，由于未成熟的胚和自然条件下没有胚乳，种子的低发芽率极大地限制了其的繁殖。在这项研究中，研究了植物生长调节剂对“ C.fabric”的体外繁殖的影响，以建立“体外”繁殖方案。结果表明，种子在2.0 mg / L N6-苄腺嘌呤（BA）+ 1.5 mg / Lα-萘乙酸（NAA）上开始形成白色斑点，最大数量为2.0 mg / L BA + 2.0 mg / L NAA。 1/2 Murashige和Skoog（MS）培养基+ 0.5 mg / L NAA是使原球茎样体（PLB）变绿的最佳培养基。在PLB的继代培养中，添加NAA的1/2 MS更有效，其生长和PLB繁殖的最佳浓度分别为2.0和1.0 mg / L。在不定芽诱导中，最佳培养基为1/2 MS + 1.0 mg / L噻唑隆（TDZ）+ 0.5 mg / L NAA，诱导率高达56.8％。此外，添加0.5 mg / L吲哚-3-丁酸（IBA）的1/2 MS是最佳生根培养基。这些结果可能为品种育种和C的大规模繁殖提供可用的技术。 faberi。

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