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首页> 外文期刊>African Journal of Biotechnology >Transformation of trehalose synthase gene (TPS Gene) into corn inbred line and identification of drought tolerance
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Transformation of trehalose synthase gene (TPS Gene) into corn inbred line and identification of drought tolerance

机译：海藻糖合酶基因（TPS基因）向玉米自交系的转化及耐旱性鉴定

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Trehalose synthase gene (TPS?gene) was transferred into maize inbred line Zheng58 with the improved pollen tube path method. At the three to five leaf stage, selection with spray Basta (0.7%) solution was conducted, and was then followed by?polymerase chain reaction?(PCR) and PCR-southern detection. The results showed that the new gene was transferred into the plants and integrated into the plant chromosomes. The initial drought-tolerance test in farmland confirmed that the drought resistance of transgenic plants was higher than that of non-transgenic plants. The results show that the new maize drought resistance germplasm can be gained by means of pollen tube path which?is?a feasible, effective and new way for maize drought resistance breeding.
机译：用改进的花粉管通道法将海藻糖合酶基因（TPSα基因）转移到玉米自交系Zheng58中。在三到五叶期，用喷雾Basta（0.7％）溶液进行选择，然后进行“聚合酶链反应”（PCR）和PCR-南方检测。结果表明，该新基因已转移到植物中并整合到植物染色体中。农田的初步耐旱性试验证实，转基因植物的抗旱性高于非转基因植物。结果表明，可以通过花粉管途径获得新的玉米抗旱种质，这是玉米抗旱育种的可行，有效和新途径。

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