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首页> 外文期刊>African Journal of Biotechnology >A new anti-lipopolysaccharide factor (EsALF-3) from Eriocheir sinensis with antimicrobial activity
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A new anti-lipopolysaccharide factor (EsALF-3) from Eriocheir sinensis with antimicrobial activity

机译：中华绒螯蟹的一种新的具有抗菌活性的抗脂多糖因子（EsALF-3）

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The cDNA of a new?Eriocheir?sinensis?ALF (designated as EsALF-3) was obtained based on EST analysis. The full-length cDNA was of 956 bp, consisting of an open reading frame (ORF) of 369 bp encoding a polypeptide of 123 amino acids. In the deduced amino acid sequence of EsALF-3, there were two highly conserved cysteine residues to define the LPS binding site, and eight positively charged residues existed between the two cysteine residues. The phylogenetic analysis revealed that EsALF-3 was clustered with the ALFs from some crustaceans, forming a separate sister branch to the group of EsALF-1 and EsALF-2 from?E.sinensis. The mRNA transcript of EsALF-3 was detected in all the examined tissues of crabs, including haemocytes, hepatopancreas, gill, muscle, heart and gonad. The recombinant protein of EsALF-3 represented antimicrobial activity against?Listonella anguillarum, Escherichia coli?and?Bacillus subtilis?with minimal?inhibitory concentration?(MIC) values of 33.75 μg mL-1, 270 μg mL-1?and 135 μg mL-1, respectively. These results together indicated that there were multiplicate ALF isoforms coexist in?E. sinensis, and the diversity of the immune effector molecules in innate immune system, such as ALF, helps Chinese mitten crab to deal with various pathogens in the aquatic environment.
机译：基于EST分析，获得了新的中华绒螯蟹ALF的cDNA（称为EsALF-3）。全长cDNA为956 bp，由369 bp的开放阅读框（ORF）组成，编码123个氨基酸的多肽。在推导的EsALF-3氨基酸序列中，有两个高度保守的半胱氨酸残基定义了LPS结合位点，并且在两个半胱氨酸残基之间存在八个带正电荷的残基。系统发育分析表明，EsALF-3与一些甲壳类动物的ALF聚集在一起，形成了中华？EsALF-1和EsALF-2的独立姊妹分支。在所有检查过的螃蟹组织中都检测到EsALF-3的mRNA转录，包括血细胞，肝胰腺、,、肌肉，心脏和性腺。 EsALF-3的重组蛋白具有抗安氏李斯特菌，大肠杆菌和枯草芽孢杆菌的抗菌活性，最小抑菌浓度（MIC）分别为33.75μgmL-1、270μgmL-1和135μgmL。 -1。这些结果一起表明在ΔE中共存在多种ALF同工型。中华绒螯蟹以及先天免疫系统中免疫效应分子（例如ALF）的多样性有助于中华绒螯蟹应对水生环境中的各种病原体。

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