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首页> 外文期刊>African Journal of Biotechnology >The study of triploid progenies crossed between different ploidy grapes
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The study of triploid progenies crossed between different ploidy grapes

机译：三倍体后代在不同倍性葡萄间杂交的研究

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The cross between different ploidy grape was one of the effective ways to obtain new seedless cultivars, in this?study, through testing the changes of the ovule weight and observing its anatomical structure, the period and mode of ovule abortion in crosses between diploid and tetraploid grape cultivars were studied, reciprocal cross was also conducted between them, the results indicated that the proper sampling period is one of the most important factors of the grape in?ovuleculture, unlike the seedless cultivars, the embryo abortion did not occur at the same time for most seeded cultivars crossed with different ploidy plants, the ovule abortion was only individual behavior. The period of ovule abortion was correlated with the mature period of the female parent. The abortive embryo of early-maturing cultivars occurred at five weeks after pollination, then the mid-maturing ones and late-maturing ones were at six and nine weeks after pollination comparatively. The reciprocal crossing results showed that this crossing form has great mating obstruction and the situation was more serious when tetraploid cultivars were used as female parent. Few seeds from 2X×4X had good germinability, while those from 4X×2X had lost their germinability and no seedlings were obtained, the highest germination rate and seedling survivals rate were obtained when the ovule was excised 60 days after pollination, it is easier to harvest hybrid progeny when using diploid as female parent.
机译：不同倍性葡萄之间的杂交是获得新的无核品种的有效途径之一，本研究通过检测胚珠重量的变化并观察其解剖结构，二倍体和四倍体杂交的胚珠流产的时期和方式。对葡萄品种进行了研究，并在它们之间进行了相互交叉，结果表明，适当的采样时间是葡萄进行无核栽培的最重要因素之一，与无核品种不同，胚芽没有同时流产对于大多数与不同倍性植物杂交的种子，胚珠流产只是个体行为。胚珠的流产期与母本的成熟期有关。早熟品种的流产胚胎发生在授粉后5周，而中熟和晚熟的流产胚胎发生在授粉后6周和9周。相互杂交的结果表明，这种杂交形式具有很大的交配障碍，当以四倍体栽培品种为母本时情况更为严重。 2X×4X的种子很少具有良好的发芽性，而4X×2X的种子失去了发芽性，没有获得幼苗，授粉后60天切下胚珠时获得了最高的发芽率和幼苗成活率。当使用二倍体作为雌性亲本时，收获杂交后代。

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