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In vitro evaluation of the interaction between methanol extract of lichen (Ramalina farinacea) and tetracycline against clinical isolates of Staphylococcus aureus

机译:地衣甲醇提取物与四环素对金黄色葡萄球菌临床分离株相互作用的体外评价

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Antimicrobial interaction studies between methanol extract of lichen (Ramalina farinacea?(I) ach. (Fam:?Ramalinacea) and tetracycline, against clinical isolates ofStaphylococcus aureus?was evaluated. Preliminary antimicrobial properties of the extract were evaluated. Antimicrobial interaction studies between the methanol extract of lichen in combination with tetracycline against strains of?S. aureus?I, J and K was carried out using checkerboard method. The preliminary antimicrobial screening revealed that the extract was effective against?S. aureus?J and K. The interaction of lichen methanol extract with tetracycline against?S.?aureus?strain I at ratios 8:2, 7:3, 6:4 and 4:6 was not significantly different, while ratios 9:1 and 3:7 showed antagonism. However, the combination ratios of methanol extract and the antibiotic against?S. aureus?J shows synergism (8:2, 7:3, 6:4, 5:5, 4:6 and 1:9), while ratios 9:1, 3:7 and 2:8 exhibit indifference. Interaction of the methanol extract and tetracycline against strain K at ratios 8:2, 6:4, 4:6 and 1:9 showed synergism, while ratios 9:1, 7:3, 5:5, 3:7 and 1:9 were not significantly different in their interactions. This shows that, in the treatment of infections caused by strains I, J and K of?S. aureus, the combination of the methanol extract of lichen (R. farinacea) and tetracycline can be used.
机译:评价了地衣甲醇提取物(Ramalina farinacea?(I)ach。(Fam:?Ramalinacea))和四环素对金黄色葡萄球菌临床分离株的抗菌作用,并对其提取物的初步抗菌性能进行了评估。甲醇之间的抗菌作用研究用棋盘法进行了地衣与四环素联合提取对金黄色葡萄球菌I,J和K菌株的抗菌作用,初步的抗菌筛选显示该提取物对金黄色葡萄球菌JJ和K的有效性。四环素对金黄色葡萄球菌菌株I的地衣甲醇提取物的比例为8:2、7:3、6:4和4:6并没有显着差异,而比例为9:1和3:7则显示出拮抗作用。甲醇提取物和抗生素对金黄色葡萄球菌?J的组合比例显示出协同作用(8:2、7:3、6:4、5:5、4:6和1:9),而比例为9:1, 3:7和2:8表现出冷漠。甲醇提取物和四氢呋喃的相互作用以8:2、6:4、4:6和1:9的比例对K菌株的细胞周期蛋白显示出协同作用,而在9:1、7:3、5:5、3:7和1:9的比例中他们的互动。这表明,在治疗由?S菌株I,J和K引起的感染中。金黄色葡萄球菌,可以使用地衣甲醇提取物和四环素的组合。

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