首页> 外文期刊>African Journal of Biotechnology >Factors influencing callus induction and plant regeneration of Dahurian wildrye grass (Elymus dahuricus L.)
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Factors influencing callus induction and plant regeneration of Dahurian wildrye grass (Elymus dahuricus L.)

机译:影响达乌里野草(Elymus dahuricus L.)愈伤组织诱导和植株再生的因素

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Establishment of highly efficient and reproducible regeneration system would greatly influence the efforts of?improvement of?Elymus?sp. through gene transfer technology. A suitable callus induction and efficient regeneration protocol for?Dahurian wildrye grass?(Elymus dahuricus L.)?was developed. It consisted of 3.0 mg/L 2,4 dichlorophenoxyacetic acid (2,4-D) or Dicamba + 1.0 g/L?casein?hydrolysate (CH) + 300 mg/L L-proline + 30 g/L sucrose in MS, and showed the highest percentage of callus induction. Highest (51%) regeneration was obtained from N6 medium containing 1.0 mg/L 2,4-D + 3.0 mg/L N6-benzylaminopurine (BA) + 1.0 g/L CH + 300 mg/ L L-proline + 30 g/L sucrose. Thus, optimization of regeneration frequency using mature seeds as explants material may offer a simple and efficient protocol forDahurian wildrye grass?that may improve molecular breeding of this species.
机译:建立高效和可再生的再生系统将极大地影响改良“ Elymus” sp的努力。通过基因转移技术。研制了一种适合于“达氏野草”(Elymus dahuricus L.)的愈伤组织诱导和高效再生方案。它由3.0毫克/升的2,4二氯苯氧基乙酸(2,4-D)或麦草畏+ 1.0克/升的酪蛋白?水解物(CH)+ 300毫克/升的L-脯氨酸+ 30克/升的蔗糖在MS中组成,并显示出最高的愈伤组织诱导率。从含有1.0 mg / L 2,4-D + 3.0 mg / L N6-苄氨基嘌呤(BA)+ 1.0 g / L CH + 300 mg / L L-脯氨酸+ 30 g /的N6培养基中获得最高(51%)再生L蔗糖。因此,以成熟种子为外植体材料优化再生频率可以为达胡尔野草提供一种简单有效的方案,从而可以改善该物种的分子育种。

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