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首页> 外文期刊>African Journal of Biotechnology >Molecular marker analysis of Shatangju and Wuzishatangju mandarin (Citrus reticulata Blanco)
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Molecular marker analysis of Shatangju and Wuzishatangju mandarin (Citrus reticulata Blanco)

机译：沙糖橘和乌子沙糖橘（Citrus reticulata Blanco）的分子标记分析

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‘Wuzishatangju’(Citrus reticulata Blanco) is an excellent cultivar derived from a bud sport of a seedy ‘Shatangju’ cultivar found in Guangdong Province in the 1980s. In this study, six molecular markers including random amplified polymorphic DNA (RAPD), inter-simple sequence repeat (ISSR), simple sequence repeat (SSR), sequence-related amplified polymorphism (SRAP), inter-retrotransposn amplified polymorphism (IRAP) and retrotransposon-microsatellite amplified polymorphism (REMAP) were used to study the genetic variations between ‘Shatangju’ and ‘Wuzishatangju’. 1196 RAPD, seven SSR, 28 IRAP and 56 REMAP primers were used to detect the genetic variations between ‘Shatangju’ and ‘Wuzishatangju’. However, no difference was observed between the two cultivars. These results indicate that there was a very close genetic relationship between ‘Shatangju’ and ‘Wuzishatangju’ and RAPD, SSR, IRAP and REMAP markers could not distinguish them. Two and 21 specific bands were obtained using 100 ISSR and 153 SRAP primers, respectively. The present research could be a valuable tool for identification of Citrus bud sport clones, which laid the foundations for the further study of the mechanisms of Citrus bud sports.
机译：“五子茶堂橘”（Citrus reticulata Blanco）是一种优良的品种，源于1980年代在广东省发现的“沙堂菊”种子的芽苗。在这项研究中，六个分子标记包括随机扩增多态性DNA（RAPD），简单序列间重复（ISSR），简单序列重复（SSR），序列相关的扩增多态性（SRAP），逆转座子之间的扩增多态性（IRAP）和使用反转录转座子-微卫星扩增多态性（REMAP）研究“沙糖菊”和“五子沙糖菊”之间的遗传变异。使用1196 RAPD，7个SSR，28个IRAP和56个REMAP引物来检测“ Shatangju”和“ Wuzishatangju”之间的遗传变异。然而，在两个品种之间没有观察到差异。这些结果表明“ Shatangju”和“ Wuzishatangju”之间存在非常密切的遗传关系，而RAPD，SSR，IRAP和REMAP标记无法区分它们。分别使用100个ISSR和153个SRAP引物获得2条和21条特定条带。本研究可能是鉴定柑橘芽运动克隆的有价值的工具，为进一步研究柑橘芽运动的机理奠定了基础。

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