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首页> 外文期刊>African Journal of Biotechnology >Reproducible in vitro regeneration system for purifying sugarcane clones
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Reproducible in vitro regeneration system for purifying sugarcane clones

机译：可再生的体外再生系统，用于纯化甘蔗克隆

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Genome purification of a selected clone of sugarcane is the key to developing homogenous lines. Generally, regenerated plants after transformation are heterogeneous at genome level, and several successive rounds of selection on antibiotic-containing medium and regeneration cycles are required to purify the genome to develop a homogenous population. Sugarcane is a vegetatively propagated plant and hence it requires to be grown in the field to harvest mature cane tops carrying meristematic tissues. In the present studies, stems of?in vitro?grownplants of four indigenous genotypes namely; HSF-242, US-778, HSF-243 and HSF-240, were subjected to regeneration. Five days post incubation at various levels of 2,4-D, the segments were placed on regeneration medium containing a combination of casein hydrolysate (500 mg/L), kinetin (0.5 mg/L) and?benzylaminopurine?(BAP, 0.5 mg/L). Response to regeneration was varied from basal to top sections. Nevertheless, more than 137 shoots were regenerated from basal segment, suggesting that the segment consisting of meristematic?tissues responded well to?in vitro?conditions. This procedure may be considered as one of the best ever published report on regeneration from?in vitro?grown plants to purify clones without subjecting the plants to field conditions and harvesting the mature cane. This technique was used to purify transgenic sugarcane plants carrying?Bacillus thuringiensis?gene.
机译：选定甘蔗克隆的基因组纯化是发展同质品系的关键。通常，转化后的再生植物在基因组水平上是异质的，需要连续几轮在含抗生素的培养基上进行选择和再生周期以纯化基因组以发展出同质种群。甘蔗是无性繁殖的植物，因此需要在田间种植以收获带有分生组织的成熟甘蔗。在本研究中，四种本地基因型的“体外”生长植物的茎分别是：使HSF-242，US-778，HSF-243和HSF-240再生。在各种水平的2,4-D下孵育五天后，将片段置于含有酪蛋白水解产物（500 mg / L），激动素（0.5 mg / L）和“苄氨基嘌呤”（BAP，0.5 mg）的组合的再生培养基上。 / L）。对再生的反应从基础到顶部各不相同。然而，从基础部分再生的芽超过137个，这表明由分生组织组成的部分对体外条件反应良好。该程序可以被认为是有史以来最好的关于从体外生长的植物再生以纯化克隆而无需使植物经受田间条件和收获成熟甘蔗的报道之一。该技术用于纯化携带苏云金芽孢杆菌基因的转基因甘蔗植物。

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