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首页> 外文期刊>African Journal of Biotechnology >An alpha-glucosidase inhibitory activity of thermostable lectin protein from Archidendron jiringa Nielsen seeds
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An alpha-glucosidase inhibitory activity of thermostable lectin protein from Archidendron jiringa Nielsen seeds

机译：id丁香种子的热稳定凝集素蛋白的α-葡萄糖苷酶抑制活性

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摘要

Inhibitors of α-glucosidase from natural resources that inhibit the digestion of carbohydrate polymers into monosaccharides in the gut are used in the treatment of insulin-independent diabetes mellitus type 2.?Archidendron jiringa?belongs to pea family of leguminous plants, some of which are a source of interesting bioactivities, including α-glucosidase inhibitory (GI) activity. A novel GI lectin was enriched from the seeds of the Djenkol bean,?A. jiringa, to apparent homogeneity by 90% saturation ammonium sulfate precipitation and Con A-Sepharose affinity column chromatography. This lectin had an IC50?value for GI activity of 0.031?±?0.02 mg/ml, an estimated molecular mass of 35.7 kDa, of which 15.8% was carbohydrate, was thermostable up to 80°C for 70 min, showed an optimum activity within the pH range of 8.0 to 10.0 and a high activity with some divalent cations such as copper (Cu2+) and high levels (50 to 100 mM) of zinc (Zn2+) and iron (Fe2+). The sequence of an internal 16 amino acid fragment of the protein showed 100% identity to the mannose-glucose specific lectin precursor of?Dioclea guainensis. The GI lectin had a high specific interaction with α-glucosidase (affinity constant = 9.3773?′?10-7?s-1,?Ks?= 0.0241 s-1,?Ka?= 2.39?′?103?s-1M-1?and?Kd?= 0.0117 M).
机译：来自自然资源的α-葡萄糖苷酶抑制剂可抑制肠道中的碳水化合物多聚体转化为单糖，可用于治疗非胰岛素依赖型2型糖尿病。花生丁香属于豌豆科豆科植物，其中一些是豌豆科植物。有趣的生物活性的来源，包括α-葡萄糖苷酶抑制（GI）活性。一种新的GI凝集素从Djenkol豆的种子中富集。通过饱和90％饱和硫酸铵沉淀和Con A-Sepharose亲和柱色谱分析发现吉林加具有明显的均质性。该凝集素的GI活性的IC50值为0.031≤±0.02 mg / ml，估计分子量为35.7 kDa，其中15.8％是碳水化合物，在80°C的温度下可稳定70分钟，表现出最佳活性。在8.0至10.0的pH范围内具有较高的活性，并且对某些二价阳离子（如铜（Cu2 +））和高水平（50至100 mM）的锌（Zn2 +）和铁（Fe2 +）具有很高的活性。该蛋白质的内部16个氨基酸片段的序列与番石榴（Dioclea guainensis）的甘露糖-葡萄糖特异性凝集素前体显示出100％的同一性。 GI凝集素与α-葡糖苷酶具有高特异性相互作用（亲和常数= 9.3773？′10 -7？s-1，？Ks？= 0.241s-1，？Ka？= 2.39？′？103？s-1M -1（ΔKd）＝ 0.0117M）。

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