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Karyotypic studies of four species of the blackfly, Simulium (Diptera: Simuliidae)

机译:four虫四种物种的核型研究(双翅目:Simuliidae)

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Karyotypic studies were carried out on the mitotic chromosomes from the supraoesophageal ganglia of four species of the blackfly,?Simulium. The four species were?Simulium alcocki?Pomeroy,?Simulium damnosum?Theobald sensu lacto and two yet unidentified species of?Simulium?herein referred to as US 1 (unidentified species 1) and US 2 (unidentified species 2). A diploid number (2n) of 6 was obtained for all the species of blackfly studied. A modified method for preparing mitotic chromosomes from the supraoesophageal ganglia was developed. This involved placing the fixed paired supraoesophageal ganglia in 2.5% hydrochloric acid for 1 to 2 s and staining in FLP orcein for 10 to 15 min. Idiograms were constructed for all the four species studied. Standard methods were adopted in the classification and nomenclature used in describing the chromosomes of the four species of blackfly in the investigation. No structural aberrations were observed in any of the species studied.
机译:核型研究是在四种食虫SiSimulium的食管上神经节的有丝分裂染色体上进行的。这四个物种是“阿尔科克”,“波莫罗伊”,“白头翁”,“ Theobald sensu lacto”和两个尚未识别的“硅”物种,在此分别称为US 1(未识别的物种1)和US 2(未识别的物种2)。对于所有研究的粉虱物种,其二倍体数(2n)均为6。开发了一种从食管上神经节制备有丝分裂染色体的改良方法。这涉及将固定的配对的食管上神经节放在2.5%的盐酸中1到2 s,然后在FLP蜡蛋白中染色10到15分钟。为所研究的所有四个物种绘制了图。在分类和命名中采用标准方法来描述研究中四种black虫的染色体。在所研究的任何物种中均未观察到结构畸变。

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