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首页> 外文期刊>African Journal of Food, Agriculture, Nutrition and Development >Bacterial Load in Expressed and Stored Breast Milk of Lactating Mothers in Abia State, Nigeria
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Bacterial Load in Expressed and Stored Breast Milk of Lactating Mothers in Abia State, Nigeria

机译：尼日利亚阿比亚州哺乳母亲表达和储存的母乳中的细菌负荷

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The use of expressed breast milk has been advocated as an effective way of encouraging and maintaining lactation when the mother is separated from the baby for a while. However, prospects of storage of expressed breast milk for any considerable period of time is hindered by the possibility of bacterial contamination and growth of infectious pathogens in the stored milk. Previous research worldwide has focused on optimal conditions for storing expressed breast milk. This study investigated bacterial growth in expressed breast milk stored at two different conditions and time periods. A total of 240 randomly selected lactating mothers participated in a survey used to elicit information on their practices concerning expressed and stored breast milk. Out of this, 20 lactating mothers were willing to donate their breast milk, which was used for microbial studies. Using antiseptic procedures, breast milk samples were collected from these 20 lactating mothers by manual expression and put into sterile containers. One half was stored at room temperature (30oC), while the other half was immersed in a container of water (26oC) for 0, 3, 6 and 9hrs. The samples at different time periods in both storage conditions were plated on three different culture media (Nutrient agar, MacConkey agar and Chocolate agar) and incubated for 24hrs. Bacterial load was enumerated and isolates were characterized and identified. Data was analyzed using one way ANOVA and presented as frequencies, percentages means and standard deviations. Results showed that in all the culture media, bacterial load was higher in breast milk samples stored at room temperature for up to 9hrs compared to that immersed in water (p<0.05). However, the average colony counts were within acceptable limits (<104CFU/ml). Enterococcus faecalis, Escherichia coli and Staphylococcus aureus were the predominant bacteria isolated in the breast milk samples under both storage conditions. The findings from this study revealed that storage of breast milk either at room temperature or immersed in a container of water appeared to be safe for up to 9 hours of storage in a tropical environment. Generally, the number of microbes was within levels considered acceptable in expressed breast milk.
机译：当母亲与婴儿分离一段时间后，已提倡使用表达乳汁作为鼓励和维持泌乳的有效方法。然而，由于存储的乳汁中细菌污染和感染性病原体生长的可能性，阻碍了在任何相当长的时间内存储表达乳汁的前景。全世界的先前研究都集中在存储表达母乳的最佳条件上。这项研究调查了在两种不同条件和时间段存储的表达母乳中的细菌生长。共有240名随机选择的哺乳期母亲参加了一项调查，以获取有关其表达和储存的母乳的做法的信息。其中，有20名哺乳期的母亲愿意捐出自己的母乳，将其用于微生物研究。使用防腐程序，通过人工表达从这20名泌乳母亲中收集母乳样品，并将其放入无菌容器中。将一半储存在室温（30oC）下，将另一半浸入水（26oC）的容器中0、3、6和9小时。将两种储存条件下不同时间段的样品铺在三种不同的培养基（营养琼脂，MacConkey琼脂和巧克力琼脂）上，孵育24小时。计数细菌载量并鉴定和鉴定分离株。使用一种方差分析对数据进行分析，并以频率，百分比平均值和标准偏差表示。结果表明，与浸入水中的细菌相比，在室温下保存长达9小时的母乳样品中的细菌载量更高（p <0.05）。但是，平均菌落数在可接受的范围内（<104CFU / ml）。在这两种储存条件下，粪肠球菌，大肠杆菌和金黄色葡萄球菌是分离出的主要细菌。这项研究的结果表明，在热带环境下，母乳在室温下或浸入水的容器中存储9小时似乎是安全的。通常，微生物的数量在表达的母乳中被认为可接受的水平内。

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