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首页> 外文期刊>African Journal of Biotechnology >Testing public Bt maize events for control of stem borers in the first confined field trials in Kenya
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Testing public Bt maize events for control of stem borers in the first confined field trials in Kenya

机译：在肯尼亚进行的首次密闭田间试验中测试公共Bt玉米事件以控制茎stem

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Transgenic? maize (Zea mays?L), developed using modified genes from the bacteriumBacillus thuringiensis?(Bt), controls stem borers without observable negative effects to humans, livestock or the environment, and is now sown on 134 million hectares globally. Bt maize could contribute to increasing maize production in Kenya. Nine public Bt maize events of?cry1Ab?and?cry1Ba?genes were tested in confined field trials site (CFTs) to assess the control of four major Kenyan stem borer species. Leaf damage rating, number of exit holes and tunnel length were scored in the field evaluations. Leaf area consumed and mortality rates among stem borers were scored in the leaf bioassays in a Biosafety Level II laboratory, located at the Kenya Agricultural Research Institute (KARI), National Agricultural Research Laboratories (NARL).? Field evaluations showed that Bt maize controlled?Chilo partellus?with mean damage scores of 1.2 against 2.7 for the non-Bt CML216 control. Laboratory bioassays showed high control for?Eldana saccharina?and?Sesamia calamistis, with mean larval mortality of 64 and 92%, respectively. However, substantial control was not observed for?Busseola fusca. These results showed that Bt maize could control three of the four major stem borers in Kenya with mortality records of 52.7% for?B. fusca, 62.3% for?E. saccharina?and 85.8% for?S. calamistis.??Additional Bt genes need to be sought and tested for effective stem borer control in all maize growing ecologies in Kenya.
机译：转基因的？玉米（Zea mays？L）是使用苏云金芽孢杆菌（Btillus thuringiensis）细菌（Bt）的修饰基因开发的，可控制bore虫，而不会对人类，牲畜或环境造成负面影响，目前已在全球1.34亿公顷的土地上播种。转基因玉米可能有助于增加肯尼亚的玉米产量。在密闭田间试验场（CFT）中测试了9个“ cry1Ab”和“ cry1Ba”基因的公共Bt玉米事件，以评估对肯尼亚四种主要stem虫的控制。在田间评估中对叶片损伤等级，出口孔数和隧道长度进行了评分。在位于国家农业研究实验室（NARL）的肯尼亚农业研究所（KARI）的生物安全II级实验室的叶片生物测定中，对茎叶虫的叶片消耗面积和死亡率进行了评分。田间评估表明，Bt玉米控制的“ Chilo partellus”平均伤害得分为1.2，而非Bt CML216对照为2.7。实验室生物测定法显示了对“ Eldana saccharina”和“ Sesamia calamistis”的高度控制，其平均幼虫死亡率分别为64％和92％。但是，未观察到Busseola fusca的实质性控制。这些结果表明，在肯尼亚，Bt玉米可以控制四个主要bore虫中的三个，其B死亡率为52.7％。 fusca，占62.3％？糖精和S的85.8％。在肯尼亚的所有玉米生长生态中，都需要寻找和测试其他Bt基因以有效控制茎bore虫。

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