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首页> 外文期刊>African Journal of Biotechnology >Kinetic growth of the isolated oleaginous yeast for microbial lipid production
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Kinetic growth of the isolated oleaginous yeast for microbial lipid production

机译：分离的含油酵母的动力学生长，可用于生产微生物脂质

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摘要

Microbial lipid was produced by the soil isolated oleaginous yeast?Torulaspora maleeae?Y30 using glucose in flask-batch fermentation. At low concentration of nitrogen, maximum lipid production rate was observed; while high nitrogen concentration (0.4 g/l) resulted in high volumetric cell mass production rate (0.960 g/l d) and specific growth rate (0.266 d-1). The isolated yeast?T. maleeae?Y30 had maximum values of 0.382 g/l d, 0.507 g lipid/g cells, 3.06 g/l and 0.105 g/L d for volumetric lipid production rate, specific yield of lipid, lipid concentration and specific rate of lipid production, respectively when culture was performed in nitrogen-limiting medium supplemented with 90 g/l glucose and 0.2 g/l (NH4)2SO4. The Monod’s constant (KS, g/l) and?μmax?(1/d) of 24.52 and 0.156, were obtained, respectively. The three major constituent fatty acids of the isolated yeast?T. maleeae?Y30 were palmitic acid, stearic acid and oleic acid that are comparable to vegetable oils, suggesting that, microbial lipid from these yeast can be used as potential feedstock for biodiesel production.
机译：在烧瓶分批发酵中，葡萄糖是通过土壤分离的含油酵母？马累螺旋藻？Y30产生的微生物脂质。在低氮浓度下，可以观察到最大的脂质产生速率。高氮浓度（0.4 g / l）导致细胞体积大批量生产速率（0.960 g / l d）和比生长速率（0.266 d-1）高。分离的酵母？马来酸？Y30的体积脂质产生速率，脂质比产量，脂质浓度和脂质产生速率的最大值分别为0.382 g / ld，0.507 g脂质/ g细胞，3.06 g / l和0.105 g / L d。当在补充有90 g / l葡萄糖和0.2 g / l（NH4）2SO4的氮限制培养基中进行培养时。得到的Monod常数（KS，g / l）和？μmax？（1 / d）分别为24.52和0.156。分离的酵母的三种主要组成脂肪酸？马来酸？Y30是与植物油相当的棕榈酸，硬脂酸和油酸，表明来自这些酵母的微生物脂质可用作生产生物柴油的潜在原料。

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