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Use of random amplified polymorphic DNA (RAPD) in differentiation of selected species of Cucumis grown in Southern India

机译:利用随机扩增多态性DNA(RAPD)区分印度南部生长的黄瓜种

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Cucumis sativus?(Cucumber),?Cucumis melo?var. acidulus?(Vellari) and?C. melo var. momordica?(Snapmelon) are three species which are widely cultivated in India as vegetable crops. These species are distinguished based on leaf, hypanthium and fruit characteristics; however,?large variation in morphological and fruit traits has been observed in different populations. A combination of morphological and random amplified polymorphic DNA (RAPD) analysis?were?carried out in these species to have a better understanding of the taxonomy. RAPD analysis of genomic DNA revealed that polymorphism with OPBE, OPBF primer series and banding pattern of OPBE-18, OPBF-03 and OPBF-15 were able to distinguish between?C. sativus,?C. melo var. acidulus?and?C. melo var. momordica.
机译:黄瓜(Cucumis sativus)(黄瓜),黄瓜(Cucumis melo)var。酸菌(Vellari)和?C。梅洛瓦苦瓜(Snapmelon)是在印度被广泛种植为蔬菜作物的三种物种。这些种类根据叶片,玻璃托和果实的特性进行区分。然而,在不同的种群中观察到形态和果实性状的巨大变化。对这些物种进行了形态学和随机扩增多态性DNA(RAPD)分析的组合,以更好地了解分类学。 RAPD分析基因组DNA表明,OPBE,OPBF引物系列的多态性和OPBE-18,OPBF-03和OPBF-15的条带模式可以区分ΔC。仙人掌梅洛瓦酸和?梅洛瓦苦瓜。

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