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Efficiency of rice straw lignocelluloses degradability by Aspergillus terreus ATCC 74135 in solid state fermentation

机译:土曲霉ATCC 74135在固态发酵中降解稻草木质纤维素的效率

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The ability of?Aspergillus terreus?for the production of cellulolytic enzymes and reduction of lignocellulose contents of rice straw in solid state fermentation was investigated in this study. Results suggested that, 8 days fermentation was appropriate, with enzymes activities as follows: FPase = 410.76 U/gDM, CMCase = 351.96U/gDM, β-glucosidase = 16.37 U/gDM, xylanase = 6166.01 U/gDM and amyloglucosidase = 425.04 U/gDM (with maximum 993.71 U/gDM on day 6). In addition, the solid state fermentation significantly (P < 0.01) reduced the concentrations of NDF, ADF, cellulose and hemicellulose in the rice straw by 19.96, 13.8, 16.32 and 32.87%, respectively. The high degradation of the hemicellulose was reflected by the high activity of xylanase enzyme, which hydrolyses xylan in hemicellulose to xylose. Higher reducing sugar and microbial cell mass productions were also obtained after 8 days fermentation. Present data showed that,?A. terreusis capable of producing high quantity of cellulolytic enzymes for the reduction of lignocellulose contents of biomass in a shorter incubation time when compared with the previously reported for biological treatment of agricultural by-products using white rot fungi.
机译:本研究研究了曲霉曲霉在固态发酵中产生纤维素分解酶和降低稻草中木质纤维素含量的能力。结果表明,适宜的发酵时间为8天,其酶活性如下:FPase = 410.76 U / gDM,CMCase = 351.96U / gDM,β-葡萄糖苷酶= 16.37 U / gDM,木聚糖酶= 6166.01 U / gDM,淀粉葡萄糖苷酶= 425.04 U / gDM(第6天的最大993.71 U / gDM)。此外,固态发酵显着(P <0.01)使稻草中的NDF,ADF,纤维素和半纤维素的浓度分别降低了19.96、13.8、16.32和32.87%。木聚糖酶的高活性反映了半纤维素的高度降解,该酶将半纤维素中的木聚糖水解为木糖。发酵8天后还获得了更高的还原糖和微生物细胞产量。目前的数据表明,与先前报道的使用白腐真菌对农业副产品进行生物处理的报道相比,土生土能够产生大量纤维素分解酶,从而在较短的培养时间内降低生物质中木质纤维素的含量。

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