• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>African Journal of Biotechnology >Protein extraction from the stem of Panax ginseng C. A. Meyer: A tissue of lower protein extraction efficiency for proteomic analysis
【24h】

Protein extraction from the stem of Panax ginseng C. A. Meyer: A tissue of lower protein extraction efficiency for proteomic analysis

机译：从人参C. A. Meyer的茎中提取蛋白质：用于蛋白质组学分析的蛋白质提取效率较低的组织

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Ginseng stem, the aerial part of the ginseng is more susceptible to diseases and environmental damages in its long process of growth. Since the molecular mechanism of why ginseng stems are vulnerable remains unclear, the comparison between healthy and pathogen suspicious tissues via proteomics approaches, such as 2-DE, could facilitate the deciphering of the pathogenesis of ginseng and improve ginseng planting industry. A major obstacle for the proteomics study of ginseng stem is the low extraction efficacy of protein due to the properties?of itsinterfering compounds. Here, we tested six different protocols of protein extraction, and identified a protocol that gave us satisfactory yield for 2-DE analysis. The protein extraction was further optimized by chloroform/isoamylol and Tris-saturated phenol extraction that reached the standard of protein purity for 2-DE.?Then, usingthe?new extraction protocol, we can efficiently analyze the protein expression patterns of ginseng stem which might provide important information for our understanding of the disease mechanism.?Also, our study would lay a foundation for the systematic analysis of the proteomics of ginseng and provide a methodological reference for other similar plant tissues.
机译：人参茎，人参的空中部分在其长期生长过程中更容易受到疾病和环境的破坏。由于尚不清楚人参茎为何易受伤害的分子机制，因此通过蛋白质组学方法（例如2-DE）比较健康人和病原体可疑组织，可以促进人参发病机理的破译，并改善人参种植业。人参茎蛋白质组学研究的主要障碍是蛋白质的提取功效低，这归因于其干扰化合物的特性。在这里，我们测试了六种不同的蛋白质提取方案，并确定了可以为我们提供令人满意的2-DE分析产率的方案。通过氯仿/异戊醇和Tris-饱和酚提取进一步优化了蛋白质的提取，达到了2-DE的蛋白质纯度标准。然后，使用新的提取方案，我们可以有效地分析人参茎的蛋白质表达模式同时，本研究将为人参蛋白质组学的系统分析奠定基础，并为其他类似植物组织提供方法学参考。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号