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首页> 外文期刊>African Journal of Biotechnology >Fructan metabolism in tall fescue calli under different environmental condition
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Fructan metabolism in tall fescue calli under different environmental condition

机译：不同环境条件下高羊茅愈伤组织中果聚糖的代谢

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Several grasses usually accumulate high levels of fructans at low temperature or in the presence of high sugar?supply. Fructans enhance plant resistance in adverse environmental conditions thanks to their vacuolar localization which allow osmotic adjustments. Tall fescue calli do not show mature vacuoles due to its anatomic characteristics, and these?in vitro?cultures can provide more knowledge about the fructan rule in unspecialized tissues especially during stress conditions. The aim of this?study?was to evaluate the sugar metabolism in embryogenic calli of tall fescue fed with different sucrose concentrations (0, 50 or 100 mM) during growth culture, kept at “control” or “cold” temperatures (23 or 4°C, respectively). In the presence of sucrose (50 or 100 mM), embryogenic calli accumulated fructans under control conditions. On the other hand, the accumulation is inhibited by the simultaneously sugar supply and cold treatment while invertase activity increased. From our data, cold temperature and high sucrose concentration seemed to be negatively correlated in the fructans biosynthetic pathway in tall fescue?in vitro?cultures.
机译：几种草通常在低温下或存在高糖供应的情况下积累高水平的果聚糖。果糖在不利的环境条件下增强了植物的抗性，这是由于它们的液泡定位可以进行渗透调节。高羊茅愈伤组织由于其解剖特征而没有成熟的液泡，这些体外培养可以提供有关非专业组织中果聚糖规则的更多知识，尤其是在压力条件下。这项研究的目的是评估在生长培养过程中饲喂不同蔗糖浓度（0、50或100 mM）的高羊茅的胚性愈伤组织中的糖代谢，并将其保持在“控制”或“冷”温度（23或4）。 °C）。在蔗糖（50或100 mM）存在下，胚性愈伤组织在对照条件下积累了果聚糖。另一方面，同时提供糖和冷处理抑制了积累，同时提高了转化酶的活性。根据我们的数据，高羊茅离体培养物中果聚糖的生物合成途径中，低温和高蔗糖浓度似乎呈负相关。

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