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首页> 外文期刊>African Journal of Biotechnology >Isolation of a novel abscisic acid stress ripening (OsASR) gene from rice and analysis of the response of this gene to abiotic stresses
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Isolation of a novel abscisic acid stress ripening (OsASR) gene from rice and analysis of the response of this gene to abiotic stresses

机译：从水稻中分离出一种新的脱落酸胁迫成熟（OsASR）基因并分析该基因对非生物胁迫的响应

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Abiotic stresses constitute a serious threat to agricultural production, which often develops into major crop production reducing factors around the world. Molecular biology technology has, however, emerged as a promising vehicle improving crop tolerance. A cold-, drought- and heat-inducible gene designated?Oryza sativa?L. abscisic acid stress-ripening (OsASR)?gene, GenBank accession: AK318549.1 was identified in rice -Pei’ai64s (O. sativa?L. ssp.?Indica?cv.) using the GeneChip rice genome array (Affymetrix) representing 51, 279 transcripts from two rice subspeciesjaponica?and?indica. The expression profile of?OsASR?obtained by the microarray analysis was confirmed by quantitative real-time?reverse transcription polymerase chain reaction (RT-PCR) analysis of the gene. The two sets of data matched very well, suggesting that?OsASR?is a multiple stresses responsive gene in rice. Based on the sequence, PCR primers were designed. The cDNA with the whole open reading frame (ORF) was amplified by PCR and cloned. Sequence analysis showed that the cDNA encodes a protein of 284 amino acid residues with M.W. ≈ 11.7 kD and pI ≈ 10.4. The gene encodes a protein with several conserved domains. Comparison of protein sequences indicates that?OsASR?encodes a putative abscisic acid stress-ripening protein. Analysis of the putative promoter region for candidate cis-regulatory elements using PlantCARE software identified seven kinds of cis-elements related to stress responses. Based on the aforementioned analyses and results obtained, we propose that?OsASR?is a novel candidate gene involved in stress tolerance in rice.
机译：非生物胁迫对农业生产构成了严重威胁，在世界范围内，非生物胁迫常常发展成主要的作物减产因素。但是，分子生物学技术已成为提高作物耐受性的有前途的载体。一种冷，干旱和热诱导基因，称为？Oryza sativa？L。使用基因芯片水稻基因组阵列（Affymetrix）在水稻-Pei'ai64s（O. sativa？L。ssp.?Indica?cv。）中鉴定到脱落酸胁迫释放（OsASR）？基因，GenBank登录号：AK318549.1两个水稻亚种japonica和indica的51、279个转录本。通过基因定量实时反转录聚合酶链反应（RT-PCR）分析证实了通过微阵列分析获得的“ OsASR”的表达谱。两组数据非常吻合，表明“ OsASR”是水稻中的多重胁迫响应基因。根据序列，设计PCR引物。通过PCR扩增具有完整开放阅读框（ORF）的cDNA并克隆。序列分析表明，该cDNA编码了284个氨基酸残基的蛋白质，M.W。≈11.7 kD，pI≈10.4。该基因编码具有几个保守结构域的蛋白质。蛋白质序列的比较表明，“ OsASR”编码一种假定的脱落酸胁迫成熟蛋白。使用PlantCARE软件对候选的顺式调控元件的推定启动子区域进行分析，确定了七种与胁迫反应相关的顺式元件。根据上述分析和获得的结果，我们提出“ OsASR”是一种参与水稻胁迫耐受性的新候选基因。

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