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首页> 外文期刊>African Journal of Biotechnology >In vitro micro-propagation of Longiflorum-Asiatic (LA) hybrids lily (Lilium) cultivar eyeliner
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In vitro micro-propagation of Longiflorum-Asiatic (LA) hybrids lily (Lilium) cultivar eyeliner

机译：Longiflorum-Asiatic（LA）杂交百合（Lilium）品种眼线的体外微繁

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摘要

Bulblets propagation by tissue culture was one of the key techniques in the production of lily (Lilium) bulbs. Therefore,?in vitro?micro-propagation of lily bulblets was studied in detail in this paper. L A hybrids lily cultivar ‘eyeliner’ was selected as the materials. By using the method of orthogonal design, the following were concluded from the research: the optimum treatment and disinfection methods of ‘eyeliner’ bulb scales was soaking in 1:500 carbendazim solution for 30 min, disinfection in 75% alcohol for 10 to 60 s, disinfection in 2% NaClO solution for 15 min; the optimum medium for bud induction of ‘eyeliner’ was MS + 0.5 mg·L-1?6-benzyl aminopurine (6-BA) + 0.1 mg·L-1naphlene acetic acid (NAA) + 90 g·L-1sucrose, and 25°C and in darkness; the optimum medium for bulblets induction of ‘eyeliner’ was 2MS + 1.0 mg·L-1?6-BA + 0.5 mg·L-1?NAA + sucrose 90 g·L-1?+ Paclobutrazol (PP333) 2 mg·L-1; the optimum culture condition for bulblets induction of ‘eyeliner’ was 20°C, 14 h·day-1?lightness + 10 h·day-1?darkness. The optimum medium for rooting culture of ‘eyeliner’ was ? MS + 0.8 mg·L-1?NAA + 3 g·L-1?activated charcoal, 20°C, 14 h·day-1?lightness + 10 h·day-1?darkness.
机译：通过组织培养繁殖的鳞茎是百合（Lilium）鳞茎生产中的关键技术之一。因此，本文详细研究了百合鳞茎的体外微繁殖。 L A杂交百合品种'眼线'被选为材料。通过正交设计的方法，得出以下结论：“眼线”球鳞的最佳处理和消毒方法是在1：500多菌灵溶液中浸泡30分钟，在75％酒精中消毒10至60 s。 ，在2％NaClO溶液中消毒15分钟;诱导眼线的最佳培养基为MS + 0.5 mg·L-1？6-苄基氨基嘌呤（6-BA）+ 0.1 mg·L-1萘乙酸（NAA）+ 90 g·L-1蔗糖，以及25°C和黑暗中;诱导球囊“眼线”的最佳培养基为2MS + 1.0 mg·L-1？6-BA + 0.5 mg·L-1？NAA +蔗糖90 g·L-1？+多效唑（PP333）2 mg·L -1;诱导“眼线笔”鳞茎的最佳培养条件为20°C，14 h·day-1？亮度+ 10 h·day-1？黑暗。培养“眼线膏”的最佳培养基是？ MS + 0.8 mg·L-1？NAA + 3 g？L-1？活性炭，20°C，14 h·day-1？亮度+ 10 h·day-1？浓度。

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