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首页> 外文期刊>African Journal of Biotechnology >Investigation of genetic effect of growth hormone gene on antler production in Chinese Sika deer Cervus nippon
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Investigation of genetic effect of growth hormone gene on antler production in Chinese Sika deer Cervus nippon

机译：生长激素基因对日本梅花鹿鹿鹿茸生产的遗传效应研究

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摘要

Antler is one of the most important products of Sika deer (Cervus nippon) and has great economic values. The genetic mechanisms and genetic markers associated with antler growth of Sika deer have been rarely reported. In the present study, the growth hormone (GH) gene was analyzed as a candidate gene for antler growth in Chinese Sika deer. Partial sequence of GH exon3 and 5 of Sika deer were obtained and four novel SNPs (ss325995760,?ss325995761, ss325995762 and ss325995763) were then identified. Genotyping of four?single nucleotide polymorphisms (SNPs)?were implemented in both?Shuangyang Sika deer (n = 69) and Dongfeng Sika deer (n = 90) using PCR-RFLP or DNA sequencing approaches, and association between different genotypes and anlter weight traits were analyzed. The SNP?ss325995760?was significantly (P < 0.05) associated with average antler yield in Shuangyang Sika deer while it was not associated in Dongfeng Sika deer. However, other three SNPs were not associated with the traits. The preliminary result implied that the identified SNPs of GH gene could be considered one of the candidate markers for marker assisted selection in Sika deer but further studies are warranted.
机译：鹿茸是西卡鹿（Cervus nippon）的最重要产品之一，具有很高的经济价值。与梅花鹿鹿角生长相关的遗传机制和遗传标记极少见。在本研究中，分析了生长激素（GH）基因作为中国梅花鹿鹿角生长的候选基因。获得了GH外显子3的部分序列和梅花鹿的5个序列，然后鉴定了四个新颖的​​SNP（ss325995760，？ss325995761，ss325995762和ss325995763）。利用PCR-RFLP或DNA测序方法，在双阳梅花鹿（n = 69）和东风梅花鹿（n = 90）中都实现了四个单核苷酸多态性（SNPs）的基因分型。性状进行了分析。双阳梅花鹿的SNPss325995760？与鹿角平均产量显着相关（P <0.05），而东风梅花鹿的SNPss325995760？与双鹿梅花鹿的平均无相关性。但是，其他三个SNP与性状无关。初步结果暗示，已鉴定的GH基因的SNP可被认为是梅花鹿中标记辅助选择的候选标记之一，但有待进一步研究。

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