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首页> 外文期刊>African Journal of Biotechnology >Inoculation of Ceratonia siliqua L. with native arbuscular mycorrhizal fungi mixture improves seedling establishment under greenhouse conditions
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Inoculation of Ceratonia siliqua L. with native arbuscular mycorrhizal fungi mixture improves seedling establishment under greenhouse conditions

机译：用天然丛枝菌根真菌混合物接种大麦角腐病可改善温室条件下的幼苗生长

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The potential benefits of inoculation with arbuscular mycorrhizal?(AM) fungi were investigated on carob tree?Ceratonia siliqua, a Mediterranean legume in Morocco. The parameters under study were the effect of an inoculation on growth, mineral nutrition and roots mycorrhizal colonization of the plant under nursery conditions.?C.?siliquagrowth was measured after six months of culture in plastic bags arranged in a randomised complete block under greenhouse conditions. Fungal inoculation consisted of a mixture of native AM fungi propagated on?Zea mays?roots. Results show that the fungal symbionts were effective to improve the growth of?C. siliqua, confirming the requirement of mycorrhizal symbiosis for the successful establishment of?C. siliqua?in a degraded soil. The approach used with indigenous AM fungi complex isolated under?C.?siliqua?appeared to be effective in promoting growth and nutrition ofC. siliqua.?After 6 months of culturing in nursery conditions, height, shoot and root biomass, total biomass, phosphorus and nitrogen foliar contents of the plants inoculated with native AM fungi were significantly higher than in the control. Glomus spores were extracted from the soil under?C. siliqua?and were observed on permanent slides under a microscope connected to a computer with digital image analysis software. Seven spore morphotypes were detected under?C. silqua?in the Ourika Valley, Morocco. Five?Glomus?species were classified as?Glomus aggregatum,Glomus intraradices and Glomus constrictum,?whereas, two other?Glomus?species were not identified. The analysis of this spore community revealed the presence of two other species belonging to?Gigaspora?genera. The use of a mixture of native AM fungi as fungal inoculum improves clearly?growth, nutrition and roots colonization of?C. siliqua?seedling.
机译：在摩洛哥的一种地中海豆类角豆树上，研究了接种丛枝菌根（AM）真菌的潜在益处。研究的参数是在苗圃条件下接种对植物生长，矿物质营养和根系菌根定殖的影响。在温室条件下以完全完整的随机排列的塑料袋中培养六个月后，测定硅纤毛的生长。 。真菌接种由在玉米根上繁殖的天然AM真菌的混合物组成。结果表明，真菌共生菌能有效地改善？C的生长。 siliqua，确认菌根共生成功建立的必要条件。硅土在退化的土壤中。在硅藻中分离出的与原生AM真菌复合物一起使用的方法看来对促进C的生长和营养有效。在苗圃条件下培养6个月后，接种天然AM真菌的植物的身高，枝条和根生物量，总生物量，磷和氮叶含量均显着高于对照。球孢子孢子是在？C下从土壤中提取的。并在与数字图像分析软件连接到计算机的显微镜下在永久性载玻片上进行观察。在？C下检测到7种孢子形态。 silqua？位于摩洛哥的欧里卡山谷。共有五个G种被分类为a小lom，intra内小and和and小Glo，而未鉴定出另外两个？种。对这个孢子群落的分析表明存在另外两个属于“ Gigaspora”属的物种。混合使用天然AM真菌作为真菌接种物可明显改善C的生长，营养和根部定植。 siliqua？种苗。

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