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首页> 外文期刊>African Journal of Biotechnology >Isolation and molecular identification of -carotene producing strains of Dunaliella salina and Dunaliella bardawil from salt soil samples by using species-specific primers and internal transcribed spacer (ITS) primers
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Isolation and molecular identification of -carotene producing strains of Dunaliella salina and Dunaliella bardawil from salt soil samples by using species-specific primers and internal transcribed spacer (ITS) primers

机译：利用物种特异性引物和内部转录间隔物（ITS）引物从盐渍土样品中分离生产杜氏盐藻和杜氏杜氏胡萝卜素的菌株并进行分子鉴定

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摘要

Dunaliella salina?and?Dunaliella bardawil?are unique species of the genus?Dunaliellathat produce large amounts of β-carotene when cultivated under appropriate conditions. These include high light intensity, high sodium chloride concentration, nitrate deficiency and extreme temperatures. Under these conditions, only?D. salinaand?D. bardawil?can accumulate β-carotene to as much as 10% of the cellular dry weight. Because the morphological characterization is based on the environmental factors, the?Dunaliella?will change the shape, so identification and differentiation ofDunaliella?species by morphology is very difficult.?In this research?study, we isolated, identified and discriminated the different?Dunaliella?β-carotene producing strains from salt soil samples, by using 18S rDNA and internal transcribed spacer (ITS) gene sequences. The soil samples were collected from four different provinces of the North Eastern part of Thailand?namely:?UdonThani, BuriRam, AmnartCharoen and Chaiyaphum.?Among the four isolates, only BuriRam KU01 and UdonThani KU01 were?D. salina?and?D. bardawil, respectively whereas AmnartCharoen KU01 and Chaiyaphum KU01 were not these?Dunaliella?species. At 4 M NaCl, with deficiency of nitrate (KNO3) and phosphate (KH2PO4) in the medium, the?D. salina?strain BuriRam KU01 produced β-carotene at the level of?56.25 ± 0.97 pg·cell-1?and?D. bardawil?strain UdonThani KU01 produced β-carotene at the level of?52.91 ± 0.29 pg·cell-1?at the 25th day after inoculation. The 18S rDNA and ITS sequences of?D. salina?strain BuriRam KU01 and?D. bardawil?strain UdonThani KU01 were submitted to the National Center for Biotechnology Information (NCBI) database with accession numbers of JN052202, JN052203, JN034031 and JN052204, respectively. By using the species-specific primers and ITS primers the β-carotene producing strains of?Dunaliella?was identified.
机译：杜氏盐藻和杜氏杜氏藻是杜氏藻的独特物种，在适当条件下培养时会产生大量β-胡萝卜素。这些包括高光强度，高氯化钠浓度，硝酸盐缺乏和极端温度。在这些条件下，仅？ salinaand？D。 bardawil？可以累积β-胡萝卜素至细胞干重的10％。由于杜氏藻的形态特征是基于环境因素而定的，因此杜氏藻的形状会发生变化，因此很难通过形态学来鉴定和区分杜氏藻。在本研究中，我们分离，鉴定和区分了杜氏藻。通过使用18S rDNA和内部转录间隔区（ITS）基因序列，从盐土样品中产生β-胡萝卜素。土壤样本是从泰国东北部的四个不同省份收集的，分别是：UdonThani，BuriRam，AmnartCharoen和Chaiyaphum。在这四个分离物中，只有BuriRam KU01和UdonThani KU01是D。盐沼和？分别是bardawil和AmnartCharoen KU01和Chaiyaphum KU01并不是这些杜氏藻。在4 M NaCl中，培养基中缺乏硝酸盐（KNO3）和磷酸盐（KH2PO4），ΔD。盐沼菌株BuriRam KU01产生的β-胡萝卜素水平为？56.25±0.97 pg·cell-1？和？D。接种后第25天，bardawil？菌株UdonThani KU01产生β-胡萝卜素，水平为52.91±0.29 pg·cell-1 ？。 ΔD的18S rDNA和ITS序列。盐沼菌株BuriRam KU01和D. bardawil？菌株UdonThani KU01已提交至国家生物技术信息中心（NCBI）数据库，登录号分别为JN052202，JN052203，JN034031和JN052204。通过使用物种特异性引物和ITS引物，鉴定了产生β-胡萝卜素的杜氏藻菌株。

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