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首页> 外文期刊>African Journal of Cellular Pathology >A comparative study of cultural and molecular techniques for the identification of bacterial contaminants of cockroaches (Periplaneta americana)
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A comparative study of cultural and molecular techniques for the identification of bacterial contaminants of cockroaches (Periplaneta americana)

机译：鉴定蟑螂细菌污染物的文化和分子技术的比较研究

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The increasing trends of insect associated bacterial infection in humans are severely hampered by disparaging number of bacteria obtained with the culture-based technique. This study therefore determined how the analysis of the 16S rDNA sequences would compare in terms of precision and reliability to the most adoptable culture-based technique. Results obtained depict enhanced accuracy of? molecular technique over the cultural method as only 249 (69%) of the total isolates were correctly identified by the cultural method to represent a total of 114 (31%)? discrepant species while 100% correct identification was observed with the molecular technique. The most predominant of these bacterial isolates from both the external surfaces and the gut environment was Escherichia coli 43 (20.8%) and 24 (15.5%) respectively. The Gram positive organisms isolated were Staphylococcus aureus, Bacillus subtilis, Staphylococcus epidermidis, and Enterococcus faecalis with a prevalence rate of 8 (3.8%), 14 (6.7%), 8 (3.8%) and 9 (4.3%) from external surfaces and 2 (1.3%), 6 (3.9%), 2 (1.3%) and 7 (4.5%) from gut environment respectively. The least isolated organisms in the external surfaces were Serratia marscencens and Citrobacter werkmanii with a distribution rate of 3(1.4%) while Citrobacter freundii 2(1.3%) was the least isolated organism from cockroach gut environment. This study therefore showed that the molecular analysis of the 16S rDNA sequences is more efficient than culture-based technique for the identification of bacterial contaminants of cockroaches because occurrences of misidentification are very much abated by this method.
机译：通过贬低通过基于培养的技术获得的细菌数量，严重阻碍了人类昆虫相关细菌感染的增长趋势。因此，这项研究确定了如何将16S rDNA序列的分析在准确性和可靠性方面与最常用的基于培养的技术进行比较。获得的结果显示出提高的准确性？分子技术优于培养方法，因为通过培养方法只能正确鉴定出总分离株的249（69％），代表总数114（31％）？分子物种可以观察到100％正确的鉴定。这些细菌从外表面和肠道环境中分离出的细菌中最主要的分别是大肠杆菌43（20.8％）和24（15.5％）。分离出的革兰氏阳性生物是金黄色葡萄球菌，枯草芽孢杆菌，表皮葡萄球菌和粪肠球菌，从外表面和外表面的患病率分别为8（3.8％），14（6.7％），8（3.8％）和9（4.3％）。肠道环境分别为2（1.3％），6（3.9％），2（1.3％）和7（4.5％）。在外表面中分离得最少的生物是粘质沙雷氏菌和韦氏柠檬酸杆菌，分布率为3（1.4％），而在蟑螂肠道环境中分离的弗氏柠檬酸杆菌2（1.3％）是分离最少的生物。因此，这项研究表明16S rDNA序列的分子分析比基于培养的技术更有效地鉴定蟑螂的细菌污染物，因为这种方法可以大大减少误识别的发生。

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