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首页> 外文期刊>African Journal of Biotechnology >Embryo rescue of crosses between diploid and tetraploid grape cultivars and production of triploid plants
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Embryo rescue of crosses between diploid and tetraploid grape cultivars and production of triploid plants

机译：二倍体和四倍体葡萄品种杂交的胚拯救和三倍体植物的生产

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Five cross combinations Jumeigui×Xinghua No.1, 87-1×Kyoho, Kyoho×Muscat Hamburg, Jumeigui× Hongqitezao and Red globle×Kyoho were used as the testing materials. Factors that affect embryo rescue from crossed seeds between diploid and tetraploid grape were studied applying L25(55) orthogonal experiment design. The effect of inoculation time, medium type and phytohormone concentration was investigated. The chromosome numbers of the progenies were identified by conventional squash method. The results show that inoculation time was the key factor that affects embryo rescue. Other factors’ effects differed according to the combination. The best inoculation time was 55 to 80 days after pollination, which lead to the highest emergence rate of 39.13% to 73.08%. The generation of triploid plants was proved by chromosome number measurement.
机译：五个交叉组合分别为聚美桂×兴化1号，87-1×巨峰，巨峰×马斯喀特汉堡，聚美桂×红旗特枣和红球×巨峰。应用L25（55）正交实验设计研究了影响二倍体和四倍体葡萄之间杂交种子胚拯救的因素。研究了接种时间，培养基类型和植物激素浓度的影响。通过常规南瓜方法鉴定后代的染色体数目。结果表明，接种时间是影响胚胎抢救的关键因素。其他因素的影响因组合而异。最佳接种时间为授粉后55至80天，导致最高出苗率达39.13％至73.08％。通过染色体数目的测量证明了三倍体植物的产生。

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