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Enhancement of Colonisation of Soybean Roots by Arbuscular Mycorrhizal Fungi Using Vermicompost and Biochar

机译:利用Ver虫和生物炭增强丛枝菌根真菌对大豆根的定殖

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Pollution and contamination of soil is one of the major concerns in the world today. Excessive use of synthetic fertilisers has caused tremendous harm to the environment and the human population indirectly. Chemical residues accumulated in crops that find their way to into the human food chain have been found to have adverse health effects. Enrichment of lakes with runoff from heavily-fertilised farms has resulted in eutrophication and pollution of water bodies. Application of organic matter and use of mycorrhiza have been recommended as ways of mitigating these problems. The aim of the study was to investigate the effect of organic amendments on colonisation of soybean roots by arbuscular mycorrhizal fungi. The study consisted of a field experiment of seven treatments with 3 replications in a complete randomized block design. The treatments were biochar (B), vermicompost (V), mycorrhiza (M), biochar and vermicompost (BV), biochar and mycorrhiza (BM), biochar, vermicompost and mycorrhiza (BVM), mycorrhiza and vermicompost (MV), and the control block which had no amendments (NT). Before planting of soybeans and application of amendments, soil samples were collected for characterization of soil chemical properties and mycorrhizal spores. Soybean seeds were planted in each plot. At flowering time, roots were screened for percentage mycorrhizal colonisation and dry mass of plants from each plot was taken. At harvest time, soil samples, plants and harvested soybeans from each plot were collected and dry weight taken. There was mean increase of 53.38% in levels of phosphorous and 15.33% of carbon in the soil after application of amendments. Levels of nitrogen decreased in all treatment blocks. There was a significant (P<0.0000) increase in arbuscular mycorrhizal fungi spores after application of treatments. The colonization percentage of arbuscules in roots was highest (14.7%) in the bio char and vermicomposting blocks (BV) while the blocks without any treatment, NT, had the lowest colonization percentage of 1.2%. The highest dry weight of both shoots and roots were recorded in blocks treated with biochar which also had the highest weight of harvested soy bean seeds with a mean of 171.28g. Blocks treated with mycorrhiza had the lowest harvest weight of soybean seeds with a mean of 58.17g. From this study it was concluded that the organic amendments enhanced the activity of the already-present mycorrhizal fungi in the soil, without requiring the introduction of commercial mycorrhizal amendments and biochar enhances microbial activity which stimulates crop productivity.
机译:土壤的污染和污染是当今世界关注的主要问题之一。过量使用合成肥料间接地对环境和人口造成了巨大伤害。已发现作物中积累的化学残留物进入人类食物链,对健康产生不利影响。高肥力农场的径流使湖泊富集,导致富营养化和水体污染。已建议使用有机物和菌根作为缓解这些问题的方法。该研究的目的是研究有机修饰剂对丛枝菌根真菌在大豆根部定植的影响。这项研究包括在完整的随机区组设计中对7种治疗方法进行3次重复的野外实验。治疗方法包括生物炭(B),ver粉(V),菌根(M),生物炭和ver粉(BV),生物炭和菌根(BM),生物炭,ver粉和菌根(BVM),菌根和ver粉(MV),以及没有修改的控制块(NT)。在种植大豆和施用改良剂之前,收集土壤样品以表征土壤化学性质和菌根孢子。在每个小区中都种植了大豆种子。在开花时,对根进行菌根定植百分比的筛选,并从每个地块中提取植物的干物质。在收获时,收集每个样地的土壤样品,植物和收获的大豆,并取得干重。施用改良剂后,土壤中的磷含量平均增加了53.38%,碳含量增加了15.33%。氮水平在所有治疗区均下降。应用处理后,丛枝菌根真菌孢子显着增加(P <0.0000)。在生物炭和ver堆砌块(BV)中,根部的丛枝定植百分比最高(14.7%),而未经任何处理的块茎(NT)的最低定植百分比为1.2%。在用生物炭处理的块中记录了茎和根的最高干重,其中也有最高收成的大豆种子重量,平均为171.28g。用菌根处理的块的大豆种子的收获重量最低,平均为58.17g。从这项研究得出的结论是,有机修饰剂增强了土壤中已经存在的菌根真菌的活性,而无需引入商业菌根修饰剂,而生物炭增强了微生物活性,从而刺激了作物的生产力。

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