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首页> 外文期刊>AIMS Genetics >In-silico based identification and functional analyses of miRNAs and their targets in Cowpea (Vigna unguiculata L.)
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In-silico based identification and functional analyses of miRNAs and their targets in Cowpea (Vigna unguiculata L.)

机译：基于计算机的Cow豆中miRNA及其靶标的鉴定和功能分析（ Vigna unguiculata L.）

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Cowpea ( Vigna unguiculata L.) is an important leguminous plant and a good diet due to presence of carbohydrate and high protein contents. Currently, only few cowpea microRNAs (miRNAs) are reported. This study is intended to identify and functionally analyze new miRNAs and their targets in cowpea. An in-silico based homology search approach was applied and a total of 46 new miRNAs belonging to 45 families were identified and functionally annotated from the cowpea expressed sequence tags (ESTs). All these potential miRNAs are reported here for the first time in cowpea. The 46 new miRNAs were also observed with stable hairpin structures with minimum free energy, ranging from ?10 to ?132 kcal mol?1 with an average of ?40 kcal mol?1. The length of new cowpea miRNAs are ranged from 18 to 26 nt with an average of 21 nt. The cowpea miRNA-vun-mir4414, is found as pre-miRNA cluster for the first time in cowpea. Furthermore, a set of 138 protein targets were also identified for these newly identified 46 cowpea miRNAs. These targets have significant role in various biological processes, like metabolism, transcription regulation as transcription factor, cell transport, signal transduction, growth & development and structural proteins. These findings are the significant basis to utilize and manage this important leguminous plant-cowpea for better nutritional properties and tolerance for biotic and abiotic stresses.
机译：pea豆（Vigna unguiculata L.）是一种重要的豆科植物，由于存在碳水化合物和高蛋白质含量，因此饮食良好。当前，仅报道了很少的cow豆microRNA（miRNA）。这项研究旨在鉴定和功能分析cow豆中的新miRNA及其靶标。应用了基于计算机内的同源搜索方法，并从the豆表达的序列标签（EST）中鉴定了属于45个家族的46种新miRNA，并在功能上进行了注释。在these豆中首次报道了所有这些潜在的miRNA。还观察到46种新的miRNA，它们具有稳定的发夹结构，具有最小的自由能，范围从？10到？132 kcal mol ？1 ，平均为40 kcal mol ？1 。新的cow豆miRNA的长度在18到26 nt之间，平均21 nt。 cow豆miRNA-vun-mir4414，首次在cow豆中被发现为pre-miRNA簇。此外，还为这些新近鉴定的46个cow豆miRNAs确定了138个蛋白质靶标。这些靶标在各种生物过程中具有重要作用，如代谢，转录调控（转录因子），细胞转运，信号转导，生长与发育以及结构蛋白。这些发现是利用和管理这种重要的豆科植物w豆以获得更好的营养特性以及对生物和非生物胁迫的耐受性的重要基础。

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