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首页> 外文期刊>American journal of applied sciences >Screening Three Strains of Pseudomonas aeruginosa: Prediction of Biosurfactant-Producer Strain | Science Publications
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Screening Three Strains of Pseudomonas aeruginosa: Prediction of Biosurfactant-Producer Strain | Science Publications

机译：筛选三株铜绿假单胞菌：生物表面活性剂生产菌株的预测科学出版物

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> Problem statement: The chemical surfactants have some disadvantages; especially, toxicity and no biodegradability. Approach: Biosurfactants were the structurally diverse group of surface-active molecules synthesize by micro-organisms. The microbial surfactants were interesting, because of the biodegradable and have many applications in industry, agriculture, medicine. Results: In the present study, the production of biosurfactant by three strains of Pseudomonas aeruginosa (PTCC 1074, 1310 and 1430) was investigated. The hemolytic and foam forming activity of different strains were studied and consequently, P. aeruginosa PTCC 1074 was selected as the suitable strain. P. aeruginosa PTCC 1074 was grown in the nutrient broth medium and biosurfactant production was evaluated every 24 h by emulsification index and surface tension for the best of production time. After that, in order to get maximum production of biosurfactant, the selected strain was grown with different additives in nutrient broth and the best culture medium was found. The biosurfactant was isolated from the supernatant and its amphipathic structure was confirmed by chemical methods. Conclusion: Biosurfactant produced by Pseudomonas aeruginosa PTCC 1074 would be considered as a suitable surfactant in industries due to its low toxicity.
机译： > 问题陈述：化学表面活性剂有一些缺点;特别是毒性和无生物降解性。 方法：生物表面活性剂是由微生物合成的结构多样的表面活性分子。微生物表面活性剂是令人关注的，因为其具有生物可降解性，并在工业，农业和医药中有许多应用。 结果：在本研究中，研究了三株铜绿假单胞菌（PTCC 1074、1310和1430）产生的生物表面活性剂。研究了不同菌株的溶血和形成泡沫的活性，因此，P。选择铜绿PTCC 1074作为合适的菌株。 P在营养肉汤培养基中培养铜绿假单胞菌PTCC 1074，并每24小时通过乳化指数和表面张力评估生物表面活性剂的生产，以期达到最佳生产时间。之后，为了最大程度地生产生物表面活性剂，将所选菌株在营养肉汤中添加不同的添加剂进行培养，从而找到最佳的培养基。从上清液中分离出生物表面活性剂，并通过化学方法证实其两亲性结构。 结论：铜绿假单胞菌PTCC 1074生产的生物表面活性剂由于其低毒性而被认为是工业上合适的表面活性剂。

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