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首页> 外文期刊>American journal of applied sciences >Spectrofluorimetric, Atomic Absorption Spectrometric and Spectrophotometric Determination of Some Fluoroquinolones | Science Publications
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Spectrofluorimetric, Atomic Absorption Spectrometric and Spectrophotometric Determination of Some Fluoroquinolones | Science Publications

机译：分光荧光法，原子吸收光谱法和分光光度法测定一些氟喹诺酮科学出版物

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> Simple, accurate, sensitive and selective spectrofluorimetric, atomic absorption spectrometric and spectrophotometric methods are described for the quantitative determination of ten fluoroquinolones (amifloxacin, ciprofloxacin hydrochloride, difloxacin hydrochloride, enoxacin, enrofloxacin, lomefloxacin hydrochloride, levofloxacin, norfloxacin, ofloxacin and pefloxacin mesylate). The first method was a spectrofluorimetric method in which samples of the studied drugs in 0.1 N H2SO4 showed native fluorescence at 450 nm when excitation was at 290 nm. The calibration graph was rectilinear from 0.3-1.4 μg mL-1 (method I). Cobalt sulphate was used for precipitation of the ion associates formed from the reaction with the cited drugs. The formation and solubility of the solid complexes at the optimum conditions of pH and ionic strength values have been studied. The method depends on direct determination of the ions in the precipitate or indirect determination of the ions in the filtrate by atomic absorption spectroscopy. The optimum conditions for precipitation were carefully studied. Rectilinear calibration graphs were obtained in the range of 3-30 μg mL-1 for each of the investigated drugs. The molar ratios of the formed chelats were determined by Job's method and their association constants were also calculated (method II). Ammonium vanadate was used for the spectrophotometric determination of the selected fluoroquinolones by oxidation in sulphuric acid medium resulting in the development of a greenish blue colour measured at 766 nm which was attributed to the vanadium (IV) produced by reduction of vanadium (V) by the selected drugs. The optimum conditions for heating time, reagent concentration and sulphuric acid concentration were carefully studied. The accuracy and precision of the proposed method was confirmed by estimating five or six replicates within Beer's law limits were obtained in the range 10-40 μg mL-1 for each of the investigated drugs with correlation coefficients not less than 0.9994 for the investigated drugs (method III). The developed spectrofluorimetric, atomic absorption spectrometric and spectrophotometric methods were applied successfully for the determination of the studied drugs in their pharmaceutical dosage forms with a good precision and accuracy compared to official and reported methods as revealed by t-and F-tests. The first method was approximately ten times more sensitive than the second and third methods. Atomic absorption spectrometric method was also applied for the determination of studied drugs in spiked urine and plasma samples.
机译： >介绍了用于定量测定十种氟喹诺酮（阿米沙星，盐酸环丙沙​​星，盐酸双氟沙星，盐酸依诺沙星，恩诺沙星，洛美沙星，降血酸诺美，左氧氟沙星，左氧氟沙星，左氧氟沙星，左氧氟沙星，左氧氟沙星，左氧氟沙星，诺氟沙星，左氧氟沙星，诺氟沙星，盐酸左氧氟沙星）的简单，准确，灵敏和选择性的荧光光谱法，原子吸收光谱法和分光光度法，氧氟沙星和甲磺酸培氟沙星）。第一种方法是分光荧光法，在0.1 N H 2 SO 4 中研究的药物样品在290 nm激发时在450 nm处显示出天然荧光。校准曲线在0.3-1.4μgmL -1 （方法I）中呈直线关系。硫酸钴用于沉淀与所引用药物反应形成的离子缔合体。已经研究了在最佳pH和离子强度条件下固体配合物的形成和溶解度。该方法取决于通过原子吸收光谱法直接测定沉淀物中的离子或间接测定滤液中的离子。仔细研究了最佳的降水条件。每种研究药物的线性校准图在3-30μgmL -1 范围内获得。通过Job方法确定所形成的螯合物的摩尔比，并计算它们的缔合常数（方法II）。钒酸铵用于通过在硫酸介质中氧化而分光光度法测定所选的氟喹诺酮类化合物，导致在766 nm处测量到呈绿色的蓝色的发展，这归因于钒（V）的还原。精选药物。仔细研究了加热时间，试剂浓度和硫酸浓度的最佳条件。通过估计每种被研究药物在10-40μgmL -1 范围内获得的啤酒定律范围内重复五到六次，从而证实了该方法的准确性和精密度。大于0.9994（方法III）。与t和F检验所揭示的官方方法和报道方法相比，已开发的分光荧光法，原子吸收光谱法和分光光度法已成功地用于确定药物剂型中研究药物的准确性和准确性。第一种方法的灵敏度大约是第二种和第三种方法的十倍。原子吸收光谱法也用于测定加标尿液和血浆样品中的研究药物。

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