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首页> 外文期刊>American journal of applied sciences >The Development of A New, Rapid, Amperometric Immunosensor for the Detection of Low Concentrations of Bacteria Part I: Design of the Detection System and Applications | Science Publications
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The Development of A New, Rapid, Amperometric Immunosensor for the Detection of Low Concentrations of Bacteria Part I: Design of the Detection System and Applications | Science Publications

机译：用于检测低浓度细菌的新型快速安培免疫传感器的开发第一部分：检测系统的设计和应用科学出版物

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> A portable, flow-through amperometric immunoassay system utilizing a newly designed immunosensor has been developed for the detection of bacteria. A sandwich scheme of immunoassay involving two stages of immunoreaction was employed using Escherichia coli as a model. E. coli antibodies were immobilized on Toray carbon paper to create a disposable immunofiltration membrane. Detection of the bacteria was achieved through the use of horseradish peroxidase-labeled antibodies, which, when coupled with a substrate of hydrogen peroxide and sodium iodide, yielded an electro-reduction, on the order of microamps, which was measured amperometrically. The immunosensor is highly sensitive and selective through the use of the disposable immunofiltration membranes that also function as immunoelectrodes, which enhance the rate of the immunoreaction due to the high surface to volume ratio of the solid to liquid phases. Potential commercialization through easier sample preparation and higher reproducibility of this system was a major objective, so the design highlights simplicity along with functionality. Preliminary data shows concentrations as low as 50 cell/mL can be detected in 22 min. Bacteria can be detected but not quantified over the desired working range of 50-1000 cell/mL. Optimization of the system parameters must be completed in order to ensure correct functionality of the sensor over the desired range of detection.
机译： >已经开发了一种利用新型设计的免疫传感器的便携式流通式安培免疫分析系统，用于检测细菌。以大肠杆菌为模型，采用了涉及两个阶段的免疫反应的免疫分析夹心方案。 E。将大肠杆菌抗体固定在Toray碳纸上，制成一次性免疫过滤膜。细菌的检测是通过使用辣根过氧化物酶标记的抗体实现的，该抗体与过氧化氢和碘化钠的底物偶联后，可产生微安量级的电还原，可通过安培法进行测量。免疫传感器通过使用也用作免疫电极的一次性免疫过滤膜而具有高度的敏感性和选择性，由于固相与液相的高表面积与体积之比，从而提高了免疫反应的速度。主要目的是通过更简便的样品制备和更高的系统重现性来实现潜在的商业化，因此设计突出了简单性和功能性。初步数据显示，可在22分钟内检测到低至50细胞/ mL的浓度。在50-1000细胞/ mL的所需工作范围内，细菌可以被检测到但无法定量。必须完成系统参数的优化，以确保传感器在所需的检测范围内具有正确的功能。

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