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首页> 外文期刊>American journal of applied sciences >Weissella cibaria Fungistatic Activity against Fusarium spp. affecting Yellow Pitahaya | Science Publications
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Weissella cibaria Fungistatic Activity against Fusarium spp. affecting Yellow Pitahaya | Science Publications

机译：Weissella cibaria对镰刀菌的抑菌活性。影响黄皮塔哈雅|科学出版物

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> >Fungistatic activity of a Lactic-Acid Bacteria (LAB) was assessed in vitro while isolated from its rumen fluid, against Fusarium oxysporum and Fusarium fujikoroi, basal-rot related species in yellow pitahaya. Fed batch was used to reproduce said LAB. Fungistatic activity kinetic tests were performed on the ferment (S1) and on two of the fractions obtained through centrifugation, supernatant (S2) and biomass (S3). The fungistatic activity was measured in two substrates, agar Man Rogosa Sharpe (MRS) and Potato Dextrose Agar (PDA). On S1 it was also performed lactic acid and acetic acid production kinetic tests as well as substrate consumption and biomass formation. Structural changes were evaluated on the fungi through microscopic electronics. The LAB was molecularly identified as Weissella cibaria. It was found that after eight hours of fermentation, fractions S1 and S3 performed the highest fungistatic activity against F. oxysporum and F. fujikoroi. Porosities were observed in the cell walls of the fungi as an effect of the LAB's fungistatic activity. These results indicate that LABs, when isolated from the animal habitat, may have a biotechnological potential to be used in the development of biocides, useful to control Fusarium in crops; for instance, to control yellow pitahaya basal rot.
机译： > >对乳酸菌（LAB）的抑菌活性进行了体外分离，从瘤胃液中分离出来，针对尖酸镰刀菌和黄皮塔哈亚的基腐相关物种 Fujiarium fujikoroi 。用补料分批繁殖所述LAB。对发酵液（S1）和通过离心获得的两个馏分，上清液（S2）和生物质（S3）进行了抑菌活性动力学测试。在两种底物琼脂Man Rogosa Sharpe（MRS）和马铃薯右旋糖琼脂（PDA）中测量了抑菌活性。在S1上，还进行了乳酸和乙酸生产动力学测试以及底物消耗和生物质形成。通过显微电子学评估真菌的结构变化。 LAB在分子上被鉴定为 Weissella cibaria 。发现发酵八小时后，馏分S1和S3对iF表现出最高的抑菌活性。 oxysporum 和 F。富士通。观察到真菌细胞壁中的孔隙是LAB抑真菌活性的影响。这些结果表明，乳酸菌从动物栖息地中分离出来时，可能具有生物技术潜力，可用于开发杀生物剂，可用于控制农作物镰刀菌。例如，控制黄色火龙果基腐病。

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