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首页> 外文期刊>American journal of applied sciences >Surface Modification of Polystyrene Beads by Ultraviolet/Ozone Treatment and its Effect on Gelatin Coating | Science Publications
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Surface Modification of Polystyrene Beads by Ultraviolet/Ozone Treatment and its Effect on Gelatin Coating | Science Publications

机译：紫外线/臭氧处理的聚苯乙烯珠的表面改性及其对明胶涂层的影响科学出版物

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> Problem statement: Polystyrene failed to provide any reactive functionality of surface hydrophilicity that is capable of binding proteins. It is known that polystyrene must be chemically modified to make its surface amenable to covalent crosslinking with protein. Approach: The aim of this study was to investigate the effects of UV/ozone treatment on gelatin coating. The surfaces of microsize polystyrene beads (150 μm) were modified by UV/ozone treatment system at different treatment time, ozone flow-rate and UV intensity was analyzed by Design expert software. The treated beads were characterized with ATR-FTIR analysis to determine the introduction of carbonyl (-C=O), carboxylic group (-COOH) and amide group (-CO-NH2) onto the polystyrene surface. Sample characterization was also carried out by SEM and densitometer. Gelatin immobilization was then preceded by incubating treated PS sample in gelatin solution and the total amount of gelatin coated on the modified surface was identified by Bradford assay. Results: The maximum amount of gelatin obtained was 63.75 μg mL-1 while the lowest amount obtained for untreated PS (9.947 μg mL-1). The introduction of carbonyl, hydroxyl and amide group on the polystyrene beads surface was confirmed by ATR-FTIR analysis and thus measures the importance of UV/ozone treatment. Conclusion: From the results, it has been found that time is the most significant factor to prepare samples for gelatin immobilization at reduced flow rate and at an increased Ultraviolet (UV) intensity in the ranges of study.
机译： > 问题陈述：聚苯乙烯无法提供任何能够结合蛋白质的表面亲水性反应性功能。已知必须对聚苯乙烯进行化学修饰以使其表面适合与蛋白质共价交联。 方法：本研究的目的是研究紫外线/臭氧处理对明胶涂层的影响。通过紫外/臭氧处理系统在不同的处理时间对微细的聚苯乙烯珠（150μm）的表面进行改性，使用Design Expert软件分析臭氧的流速和紫外强度。通过ATR-FTIR分析对处理过的珠子进行表征，以确定在聚苯乙烯表面上引入了羰基（-C = O），羧基（-COOH）和酰胺基（-CO-NH 2 ） 。样品表征也通过SEM和光密度计进行。然后在明胶固定之前，将处理过的PS样品在明胶溶液中孵育，然后通过Bradford分析鉴定涂覆在改性表面上的明胶总量。 结果：获得的最大明胶量为63.75μgmL -1 ，而未处理的PS的最低量为9.947μgmL -1 ） 。通过ATR-FTIR分析证实了在聚苯乙烯珠粒表面上引入了羰基，羟基和酰胺基，从而测量了UV /臭氧处理的重要性。 结论：从结果中发现，在研究范围内，时间是制备明胶固定样品的最重要因素，明胶固定样品的流速应降低，紫外线强度应提高。

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