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首页> 外文期刊>American journal of applied sciences >BIO-GUIDED STUDY ON MELASTOMA MALABATHRICUM LINN LEAVES AND ELUCIDATION OF ITS BIOLOGICAL ACTIVITIES | Science Publications
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BIO-GUIDED STUDY ON MELASTOMA MALABATHRICUM LINN LEAVES AND ELUCIDATION OF ITS BIOLOGICAL ACTIVITIES | Science Publications

机译：拟南芥无性系叶子的生物引导研究及其生物学活性的测定科学出版物

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> The present study aimed to isolate and identify constituents with antibacterial activity from the Methanol extract of Melastoma malabathricum leaves (MMML) through bioassay guided fractionation. Furthermore, the study scoped to evaluate the cytotoxic effects of M. malabathricum leaves fractions for possible protective effect of antoxidative constituents present in the fractions. The MMML extract was fractionated by Vacuum Liquid Chromatography (VLC) to afford M. malabathricum leaves fractions (ML1-ML6) of increasing polarities. Antibacterial activity of plant extract, six fractions and two bioactive constituents against Staphylococcus aureus reference strain, methicillin-resistant Staphylococcus aureus reference strain (MRSA), eleven clinical MRSA isolates, three clinical Pseudomonas aeruginosa isolates and P. aeruginosa reference strain was evaluated using Minimum Inhibitory Concentration (MIC). The bioactive constituents responsible for antibacterial activity of MMML, fractions were detected by direct TLC-bioautography. The data obtained from MIC assay showed that ML5 effectively inhibited growth of all test bacterial pathogens. Direct TLC-bioautography revealed that ML5 had the highest number of antibacterial compounds. Following bioassay-guided fractionation, Kaempferol-3-O-(2?, 6?-di-O-p-trans-coumaroyl)-?-glucopyranoside and Kaempferol were isolated from ML5. The structures of bioactive compounds were elucidated using Nuclear Magnetic Resonance (NMR) spectroscopy from 1HNMR and 13CNMR. In addition, the cytotoxic effect of M. malabathricum leaves fractions (ML1-ML6) revealed that ML5 had the highest Cytotoxicity Concentration (CC50) at 0.75 mg mL-1, an observation traceable to the presence of flavonoids constituents with antioxidant properties. Therefore, the results clearly indicate that MMML, fractions and bioactive constituents have profound antibacterial activity. Furthermore, antioxidative compounds from ML5 fraction exert their effects by enhancing the level of CC50.
机译： >本研究旨在通过生物测定引导分级分离法从 Malastathricum 叶片的甲醇提取物中分离和鉴定具有抗菌活性的成分。此外，该研究旨在评估 M的细胞毒性作用。马拉巴利姆叶留有级分，以保护级分中存在的抗氧化成分。 MMML提取物通过真空液相色谱法（VLC）分级分离，得到M。灵长类动物留下的极性增加的级分（ML1-ML6）。植物提取物，六种组分和两种生物活性成分对金黄色葡萄球菌参考菌株，耐甲氧西林的金黄色葡萄球菌参考菌株（MRSA），十一株临床MRSA分离株，三株临床抗菌活性铜绿假单胞菌和 P。使用最小抑制浓度（MIC）评估铜绿参比菌株。通过直接TLC-生物自显影术检测负责MMML抗菌活性的生物活性成分。从MIC测定获得的数据表明ML5有效抑制了所有测试细菌病原体的生长。直接TLC生物自显影显示ML5具有最高数量的抗菌化合物。在生物测定指导的分级分离之后，Kaempferol-3- O -（2α，6α-di- Op-trans -香豆酰基）-？从ML5中分离了β-吡喃葡萄糖苷和山奈酚。利用 1 HNMR和 13 CNMR，利用核磁共振波谱法阐明了生物活性化合物的结构。另外，M的细胞毒性作用。马拉叶的叶级分（ML1-ML6）显示ML5在0.75 mg mL -1 50 ） >，可追溯到具有抗氧化特性的类黄酮成分的观察。因此，结果清楚地表明，MMML，级分和生物活性成分具有深远的抗菌活性。此外，ML5馏分中的抗氧化化合物通过提高CC 50 的水平发挥作用。

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