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首页> 外文期刊>American Journal of Analytical Chemistry >A Validated Inductively Coupled Plasma-Optical Emission Spectrometry (ICP-OES) Method to Estimate Free Calcium and Phosphorus in In Vitro Phosphate Binding Study of Eliphos Tablets
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A Validated Inductively Coupled Plasma-Optical Emission Spectrometry (ICP-OES) Method to Estimate Free Calcium and Phosphorus in In Vitro Phosphate Binding Study of Eliphos Tablets

机译：验证的感应耦合等离子体发射光谱法（ICP-OES）估计Eliphos片剂的体外磷酸盐结合研究中的游离钙和磷

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摘要

An ICP-OES method has been developed to estimate Calcium and Phosphorous in In vitro phosphate binding study of Eliphos Tablets. The method is selective and is capable of detecting calcium and phosphorous in the presence of other trace elements. The method has been validated using RF power of 1500 watts, plasma flow of 15L/min, Nebuliser flow of 0.8 L/min and plasma view at radial mode for calcium and axial mode for phosphorus. The wavelength was monitored for calcium and phosphorous at 317.933 nm and 213.677 nm respectively. The method has been validated in terms of specificity, precision, linearity, accuracy, limit of quantification and ruggedness. The In vitro binding studies were performed for Eliphos Tablets at eight dif- ferent phosphate concentrations by incubating at 37.0?C and analysis was performed using the validated method to estimate free calcium and phosphorus. The objective of the study is to provide an alternate In vitro method to estimate the binding capacity of calcium acetate tablets to avoid the expensive in-vivo bio clinical studies.
机译：已开发出一种ICP-OES方法来估算Eliphos片剂的体外磷酸盐结合研究中的钙和磷。该方法是选择性的，并且能够在其他微量元素存在的情况下检测钙和磷。使用1500瓦的RF功率，15 L / min的血浆流量，0.8 L / min的雾化器流量以及钙的径向模式和磷的轴向模式对方法进行了验证。分别在317.933 nm和213.677 nm处监测波长的钙和磷。该方法已在特异性，精密度，线性，准确性，定量限和耐用性方面得到验证。通过在37.0？C下孵育，在8种不同磷酸盐浓度下对Eliphos片剂进行了体外结合研究，并使用经过验证的方法进行了分析以估计游离钙和磷。该研究的目的是提供一种替代的体外方法来评估醋酸钙片的结合能力，从而避免进行昂贵的体内生物临床研究。

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