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首页> 外文期刊>American Journal of Analytical Chemistry >Quantitative Determination of Nitidine from Roots and Plant Tissue Culture Extracts of Toddalia asiatica (Linn.) Using HPTLC
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Quantitative Determination of Nitidine from Roots and Plant Tissue Culture Extracts of Toddalia asiatica (Linn.) Using HPTLC

机译：使用HPTLC定量测定虎杖（Toddalia asiatica（Linn。））根和植物组织培养提取物中的亚硝胺含量

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摘要

Toddalia asiatica Linn. is an important medicinal plant belonging to the family Rutaceae. The plant is well known for its antimalarial activity, which has been attributed to the presence of benzophenanthridine alkaloid nitidine in the roots of plants. A simple, rapid, sensitive, accurate, repeatable and robust HPTLC method has been developed and validated for the quantitative determination of nitidine in the dried roots and plant tissue culture extracts of T. asiatica. Nitidine was estimated at 332 nm by densitometry using Silica gel 60 F254 as stationary phase and chloroform:methanol (7:1, v/v), and as mobile phase. Linearity was observed in the concentration range of 25 -200 ng/spot for nitidine. The limit of detection and limit of quantitation were found to be 0.026 and 0.086 ng/spot respectively for nitidine. Developed method was validated according to the ICH guidelines with respect to precision, accuracy, specificity and robustness. The technique has been applied for the first time for the estimation of nitidine in roots and plant tissue culture extracts of T. asiatica. Statistical analysis data indicate the accuracy and reliability of the method.
机译：Toddalia asiatica Linn。是属于芸香科的重要药用植物。该植物因其抗疟活性而闻名，这归因于植物根部存在苯并菲啶生物碱亚硝胺。已经开发了一种简单，快速，灵敏，准确，可重复且可靠的HPTLC方法，并已用于定量测定亚洲锥果干根和植物组织培养提取物中的亚尼替丁。使用硅胶60 F254作为固定相和氯仿：甲醇（7：1，v / v）作为流动相，通过光密度法在332 nm处估算出亚硝胺。在亚尼替丁的浓度范围为25 -200 ng / s处观察到线性。发现亚尼替丁的检测极限和定量极限分别为0.026和0.086 ng / spot。根据ICH指南对开发的方法进行了准确性，准确性，特异性和鲁棒性验证。该技术已首次应用于估算亚洲锥果根和植物组织培养提取物中的亚硝胺。统计分析数据表明了该方法的准确性和可靠性。

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