首页> 外文期刊>American Journal of Drug Discovery and Development >Effect of Host Plant on Gut Physicochemistry and Digestive Enzymes of the Cutworm ( Agrotis ipsilon ) Larvae
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Effect of Host Plant on Gut Physicochemistry and Digestive Enzymes of the Cutworm ( Agrotis ipsilon ) Larvae

机译:寄主植物对地老虎(Agrotis ipsilon)幼虫肠道生理生化和消化酶的影响。

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The effect of nutrition on different host plants (cabbage, castor bean and cotton) on midgut redox potentional (Eh), midgut pH and digestive enzymes of the fourth larval instar of Agrotis ipsilon was studied. The results showed that all larvae had strongly alkaline midguts pH ranging from 9-9.75. There were no significant differences between midgut pH of larvae fed for 24 h or that fed for 7 days, effect different host plants, reflecting the rapidity by which the larvae adjust their midgut pH. On the contrary, plant species had a significant effect on mid gut Eh. Conditions in all guts were oxidizing ranging from +171 to +250 mV i.e., all tested midguts Eh were positive. The results also revealed that continuous feeding on host, plants for 7 days significantly lowered the oxidizing state of gut, except cabbage, as compared to those larvae fed for 24 h. Each host plant had own specific effect on protease, lipase and invertase. However lipase was the least enzyme that affected. The study clarifies the effect of host plants on midgut physicochemistry which mid gut affect digestion and gut physiology, ultimately affecting the normal growth of the pest.
机译:研究了营养对不同寄主植物(卷心菜,蓖麻子和棉花)的营养作用,对农杆菌四龄幼虫的中肠氧化还原潜能(Eh),中肠pH和消化酶的影响。结果表明,所有幼虫均具有强碱性中肠,pH范围为9-9.75。喂食24 h或喂食7天的幼虫中肠pH值之间没有显着差异,影响不同的寄主植物,反映了幼虫调节中肠pH的快速性。相反,植物物种对中肠Eh有显着影响。所有肠道的氧化状况为+171至+250 mV,即所有测试的中肠Eh均为阳性。结果还表明,与饲喂24小时的幼虫相比,连续饲喂寄主植物7天可显着降低除卷心菜以外的肠道氧化态。每个宿主植物对蛋白酶,脂肪酶和转化酶都有自己的特异性作用。然而,脂肪酶是受影响最小的酶。该研究阐明了寄主植物对中肠生理化学的影响,中肠影响消化和肠道生理,最终影响害虫的正常生长。

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