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首页> 外文期刊>American Journal of Drug Discovery and Development >Cellular Mechanism of the Cytotoxic Effect of Extracts from Syzygium polyanthum Leaves
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Cellular Mechanism of the Cytotoxic Effect of Extracts from Syzygium polyanthum Leaves

机译：桔梗叶提取物的细胞毒作用的细胞机制

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The research objective is to obtain the cellular mechanism(s) of Syzygium polyanthum leaves for its development into commercial herbal product for certain degenerative diseases. The approach was to conduct activity-guided fractionation of metabolite extracts based on the antioxidant and the apoptotic modulation activities using cultured cells. Antioxidant activity of the active fractions were screened using 1,1-diphenyl-2-picrylhydrazil (DPPH) free radical assay and thiobarbituric acid (TBA) assay. The cellular effects were investigated using cultures of HB4C5 human hybridoma and mouse colon 26 adenocarcinoma cells. The flavonoids extract which has the lowest IC50 value of 14.84 μg mL-1 for DPPH assay among other metabolites, was found to have significant cytotoxic effect on both HB4C5 and colon 26 cells. Specific assays for apoptosis using caspase 3-gene expression and annexin-V flow cytometry analyses, supported the effect of cell proliferation inhibition which was not due to apoptosis. Subsequent cell cycle analysis indicated that crude extract may induce cell cycle arrest of HB4C5 cells at G1 to S phase, whereas active compound fractions of flavonoids stacked the cell cycle at G2/M phase. These observations suggest that flavonoid extracts of Syzygium polyanthum posses active compound(s) with anti-proliferative effect. This is a new finding for therapeutic potential of Syzygium polyanthum leaves in Indonesian traditional medicines.
机译：该研究目的是获得蒲桃叶片的细胞机制，以使其发展成用于某些退行性疾病的商业草药产品。该方法是使用培养的细胞基于抗氧化剂和细胞凋亡调节活性进行代谢物提取物的活性指导的分级分离。活性级分的抗氧化活性使用1，1-二苯基-2-吡咯肼（DPPH）自由基测定法和硫代巴比妥酸（TBA）测定法进行筛选。使用HB4C5人杂交瘤细胞和小鼠结肠26腺癌细胞培养物研究了细胞效应。黄酮类提取物在其他代谢产物中的DPPH测定的IC50值最低，为14.84μgmL-1，被发现对HB4C5和结肠26细胞均具有明显的细胞毒性作用。使用caspase 3-基因表达和膜联蛋白-V流式细胞术进行凋亡的特异性测定，支持了细胞增殖抑制的作用，这不是由于凋亡引起的。随后的细胞周期分析表明，粗提取物可能诱导HB4C5细胞在G1到S期的细胞周期停滞，而类黄酮的活性化合物部分在G2 / M期使细胞周期堆积。这些观察结果表明，蒲黄的类黄酮提取物具有抗增殖作用的活性化合物。这是在印尼传统医学中发现蒲桃叶片治疗潜力的新发现。

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