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首页> 外文期刊>American Journal of Microbiology >Isolation of Amylolytic Bacteria from Viscera of Labeo rohita (Ham.) and Optimization of Alpha Amylase Extraction | Science Publications
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Isolation of Amylolytic Bacteria from Viscera of Labeo rohita (Ham.) and Optimization of Alpha Amylase Extraction | Science Publications

机译：Labeo rohita（Ham。）内脏中淀粉分解菌的分离及α淀粉酶提取的优化|科学出版物

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> Problem statement: Fish is largely consumed in the north-eastern part of India resulting in the generation of huge amount of fish viscera in the market places. These waste generated is not properly managed for disposal. Under such circumstances it is necessary to properly utilize this waste regenerating the possibility of environment friendly disposal. Besides, industrially and commercially useful biological products like enzymes can be obtained from these sources which, therefore, can benefit the human society through proper exploitation utilizing modern technological innovations. Although there are well established commercially viable protocols for large scale production of α- amylase, but it is a known fact that it requires extensive infrastructure including microbial culture laboratory and large scale processes for providing optimum conditions to industrially important microbes for the production of enzymes at an industrial scale. This requires huge expanses in terms of money and time. Approach: In the present investigations, we report the development of optimized parameters for the extraction of α-amylase from the viscera of Labeo rohita (Ham.) after isolation and screening of amylolytic bacteria from the viscera of Labeo rohita (Ham.). The potent amylolytic bacterial isolate was subjected to solid substrate fermentation to enhance the production of α-amylase. Results: Optimization of different parameters during solid state fermentation enhanced the expression of the enzyme activity from 24.75-60.2 Unit g−1. Out of 15 amylolytic bacterial isolates obtained in the following studies, Bacillus megaterium showed the highest amylolytic activity (11 Unit g−1) when wheat bran was used as the carbon substrate during fermentation for 5 days at 37°C. Conclusion: This report support the proper use of human generated biological wastes for developing low cost technology in producing commercially viable and economically important biological molecules like enzymes for human benefit.
机译： > 问题陈述：在印度东北部，鱼类大量消费，导致市场上产生大量的鱼类内脏。这些产生的废物没有得到适当处理以进行处置。在这种情况下，有必要适当利用这些废物，以重新产生对环境友好的处置方法。此外，可以从这些来源获得工业和商业上有用的生物产物，如酶，因此，通过利用现代技术创新进行适当的开发，可以使人类社会受益。尽管已经建立了可大规模生产α-淀粉酶的商业可行方案，但众所周知的事实是，它需要广泛的基础设施，包括微生物培养实验室和大规模工艺，以便为工业上重要的微生物提供最佳条件以生产酶。工业规模。这需要大量的金钱和时间。 方法：在目前的研究中，我们报告了分离和筛选出从 Labeo rohita （Ham。）内脏中提取α-淀粉酶的优化参数的开发。来自 Labeo rohita （Ham。）内脏的淀粉分解菌。将有效的淀粉分解细菌分离物进行固体底物发酵以增强α-淀粉酶的产生。 结果：固态发酵过程中不同参数的优化提高了酶活性的表达，从24.75-60.2单位g -1 。在以下研究中获得的15种淀粉分解细菌分离物中，当麦麸用作碳底物时，巨大芽孢杆菌显示出最高的淀粉分解活性（11单位g -1 ）。在37°C下发酵5天。 结论：本报告支持人为产生的生物废物的正确使用，以开发低成本技术，以生产对商业有益的，具有经济意义的重要生物分子，如对人类有益的酶。

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