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首页> 外文期刊>American Journal of Microbiology >Strains of Klebsiella Pneumoniae from Intensive Care Unit Producing CTX-M-15 Extended Spectrum Beta Lactamases | Science Publications
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Strains of Klebsiella Pneumoniae from Intensive Care Unit Producing CTX-M-15 Extended Spectrum Beta Lactamases | Science Publications

机译：重症监护病房生产CTX-M-15广谱β内酰胺酶的肺炎克雷伯菌菌株科学出版物

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Problem statement: Resistance to third generation cephalosporins due to acquisition and expression of Extended Spectrum β-Lactamase (ESBL) among Gram-negative bacteria is on the increase. Infections involving extended spectrum beta lactamase bacteria are associated with significant morbidity and mortality. Therefore, infections due to ESBL isolates continue to pose a serious challenge to infection management worldwide. Since screening for ESBL is not a common practice in hospitals in Enugu state, this study was undertaken to characterize ESBL genes in K. pneumoniae strains from intensive care unit of the University of Nigeria Teaching Hospital (UNTH) Enugu. Approach: Over a period of 29 months, 57 patients out of 140 receiving treatment in the intensive care unit of the University of Nigeria Teaching Hospitals Enugu were found to be infected with extended-spectrum β-lactamase-producing strains of K. pneumoniae. Species identification of K. pneumoniae strains was performed by Standard Microbiology methods and re-confirmed by MALDI-TOF technology. Phenotypic characterization of Extended Spectrum Beta Lactamase (ESBL) was determined by double disc synergy test and presence of ESBL genes was determined by specific PCR. Results: All ESBL producers were positive in a PCR for blaCTX-M-1 cluster and on sequencing, blaCTX-M-15 were found to be present. Genotypic characterization of extended spectrum beta-lactamase producing K. pneumoniae showed that all isolates carried CTX-M-15and SHV genes, 41(71.9%) carried aac (6
机译：问题陈述：由于革兰氏阴性细菌中扩展谱-内酰胺酶（ESBL）的获得和表达，对第三代头孢菌素的耐药性正在增加。涉及广谱β-内酰胺酶细菌的感染与明显的发病率和死亡率有关。因此，由于ESBL分离株引起的感染继续对全世界的感染管理构成严峻挑战。由于在Enugu州的医院中筛查ESBL并非普遍做法，因此本研究旨在表征来自尼日利亚大学教学医院（UNTH）Enugu的重症监护病房的肺炎克雷伯菌菌株中的ESBL基因。方法：在29个月的时间里，发现尼日利亚140名在埃努古大学教学医院的重症监护病房接受治疗的140名患者中有57名患者感染了产生广谱＃-内酰胺酶的K株。肺炎肺炎克雷伯氏菌菌株的物种鉴定通过标准微生物学方法进行，并通过MALDI-TOF技术再次确认。通过双盘协同试验确定超广谱β-内酰胺酶（ESBL）的表型特征，并通过特异性PCR确定ESBL基因的存在。结果：所有ESBL生产者在blaCTX-M-1簇的PCR中均为阳性，在测序中发现blaCTX-M-15。产超广谱β-内酰胺酶的肺炎克雷伯菌的基因型鉴定表明，所有分离株均携带CTX-M-15和SHV基因，其中41个（71.9％）携带aac（6

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