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首页> 外文期刊>American Journal of Microbiology >Fungal Concentrations in Dust Samples of Animal Rearing Houses of Bangalore, India | Science Publications
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Fungal Concentrations in Dust Samples of Animal Rearing Houses of Bangalore, India | Science Publications

机译：印度班加罗尔动物饲养场灰尘中的真菌浓度科学出版物

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> >Fungal concentrations in dust samples analyzed by molecular methods were carried out of animal rearing house at Hessaraghatta village, Bangalore. Handy air sampler EnviroTech-APM 821 was used to collect the dust samples in triplicate and sampling time was limited to eight hours. The sampled filter papers were used for the assay of DNA extracted from all the four animal rearing locations were used for PCR amplification using Universal ITS fungal identification primers PCR products were analyzed on 2% agarose gel out of four different locations were rabbit house, cow shed, poultry farm and swine house. The sequencing was then blasted against known sequences in the NCBI database to provide species identification and phylogenetic tree was also constructed with 20 closely related blast sequences. The rabbit house and cow shed dust samples did not show any amplifications even with varying conditions while poultry farm (Cladosporium oxysporium DFFSCS018) and swine house (Peniophora species1306-hpF-04) isolate samples gave amplicons of 550bp and 600bp respectively with ITS region showing 99% similarity. These studies useful for clinicians to identify the cause of the dust allergic diseases of the animal rearing house workers and suggest amendment strategies.
机译： > >通过分子方法分析的灰尘样品中的真菌浓度是在班加罗尔Hessaraghatta村的动物饲养场进行的。便携式空气采样器EnviroTech-APM 821用来一式三份收集灰尘样品，采样时间限制为八小时。采样的滤纸用于分析从所有四个动物饲养场所提取的DNA，并使用Universal ITS真菌鉴定引物进行PCR扩增在2个琼脂糖凝胶上分析PCR产物的四个不同位置，分别是兔房，牛棚，家禽农场和养猪场。然后针对NCBI数据库中的已知序列对序列进行原始序列分析，以提供物种鉴定，并使用20个紧密相关的原始序列构建系统树。兔舍和牛棚粉尘样品即使在不同条件下也不会显示任何扩增，而家禽场（Cladosporium oxysporium DFFSCS018）和猪舍（Peniophora种1306-hpF-04）分离样品分别产生了550bp和600bp的扩增子，ITS区域显示99 ％相似度。这些研究对于临床医生识别动物饲养工人的粉尘过敏性疾病的原因并提出修正策略很有用。

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