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首页> 外文期刊>American Journal of Microbiology >Extended-Spectrum β-Lactamases Producing Escherichia coli Strains Monitored Over 4 Years in The University Hospital in Košice, Slovakia | Science Publications
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Extended-Spectrum β-Lactamases Producing Escherichia coli Strains Monitored Over 4 Years in The University Hospital in Košice, Slovakia | Science Publications

机译：斯洛伐克科希策大学医院对产大肠埃希菌菌株的广谱β-内酰胺酶进行了4年监测科学出版物

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> >Thebeta-lactamases with extended spectrum of activity (ESBL) are medically one ofthe most important group of enzymes. The presented study providesidentification and determination of the spectrum of resistance againstdifferent and clinically used antimicrobial drugs in the clinical isolates of Escherichiacoli. These isolates had their origin in different departments of theUniversity Hospital L. Pasteur in Košice. The second goal was the detection ofbeta-lactamase production with extended-spectrum effect (ESBL) and testing ofAmpC-type cephalosporinases by several phenotypic tests in clinical isolates.We used both the microdilution method and the method with an active agent,respectively. Samples were positively tested for ESBL with the use of the CLSIdisk diffusion method. PCRs were performed with a series of primers designedfor the detection of Ambler class A, B and C beta-lactamase genes. About 307strains of E. coli were investigated. The growth of E. coliresistance to selected antibiotics was present in 83.25% of clinical isolates.There were identified 85 positive isolates in the studied group and the prevalence of the ESBL positive strains ofE. coli reached 27.78%. An E. coli strain was isolated withmutations in the promoter region of the AmpC chromosomal gene that isassociated with overproduction of the relevant enzyme. We describe a complexESBL epidemiology. The study revealed a high rate of ESBL-producing E. coliisolates. The blaTEM and blaSHV enzymesdominated in ESBL-positive E. coli isolates in the University HospitalL. Pasteur in Košice.
机译： > >具有广泛活性的β-内酰胺酶（ESBL）在医学上是最重要的酶之一。本研究提供了对临床分离的埃希氏菌的不同和临床使用的抗菌药物的耐药谱的鉴定和测定。这些分离株起源于科希策的L. Pasteur大学医院的不同部门。第二个目标是通过临床分离物中的几种表型测试来检测具有广谱效应（ESBL）的β-内酰胺酶的产生并通过多种表型测试来检测AmpC型头孢菌素酶。使用CLSIdisk扩散方法对样品进行ESBL阳性测试。用一系列设计用于检测Ambler A，B和C类β-内酰胺酶基因的引物进行PCR。约307株E。对大肠杆菌进行了调查。 E的增长。临床分离株中83.25％存在对选定抗生素的耐药性。在研究组中鉴定出85株阳性分离株和ESBL阳性ES株的流行。大肠杆菌达到27.78％。一个 E。分离出带有相关酶过量产生的AmpC染色体基因启动子区突变的大肠杆菌菌株。我们描述了一种复杂的ESBL流行病学。该研究表明产生ESBL的E的比率很高。大肠杆菌分离株。 ESBL阳性 E中以 bla TEM 和 bla SHV 酶为主。大学医院的大肠杆菌分离株。巴斯德在科希策。

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