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首页> 外文期刊>American Journal of Plant Sciences >In Vitro Evaluation of Fasciolicide Activity with Hexane, Methanol and Ethyl Acetate with Extracts Processed and Obtained from Some Mexican Plants Used in Traditional Medicine Based on Ethno Botanical Studies
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In Vitro Evaluation of Fasciolicide Activity with Hexane, Methanol and Ethyl Acetate with Extracts Processed and Obtained from Some Mexican Plants Used in Traditional Medicine Based on Ethno Botanical Studies

机译：基于民族植物学研究，从墨西哥一些用于传统医学的植物中提取得到的提取物，对己烷，甲醇和乙酸乙酯中的杀草剂活性进行了体外评估

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Fasciolosis is a parasitic disease of world distribution affecting mainly domestic ruminants. The control of this disease is carried out using chemical fasciolicides which, in some cases, have been observed to have environmental problem such us pollution resistance. An alternative is to investigate extracts from plants with anti-Fasciola hepatica effects, taking advantage of the great diversity of flora of our country. The aim of this paper is to identify, assess and elucidate the anti-Fasciola hepatica effect in vitro using antiparasitic extracts of some plants used in Mexican ethno botany. We collected, dried, processed and tested in vitro about 20 plants with their respective chemical elements (hexane, ethyl acetate and methanol), obtaining results of a total of 60 extracts tested. The in vitro evaluations were carried out for 3 days, and the efficacy of each extract was compared with an untreated control group. Each test was repeated 6 times with 13 extracts that showed greater fasciolocide activity. Results from these 13 extracts tested ranged from 80% to 100% activity and the plants tested were: Castela tortuosa (chaparro amargo), Achillea millefolium (plumajillo), Thymus vulgaris (thyme), Justicia spicigera (muicle), Limpia critridora (cedron), Populus alba (Alamo), Mentha piperita (mint), Chenopodium graveolens (epazote de zorrillo), Lippia graveolens (oregano), Artemisia mexicana (estafiate) and Artemisia absinthium (wormwood), which is the hexane variable which showed higher fasciolicide capacity; using a dose of 500 gr/Lt in all the trials. Further in vitro studies should be conducted to obtain the LD50 of each extract to be able to isolate the main active element found in the hexane variable.
机译：筋膜病是一种世界范围内的寄生虫病，主要影响国内反刍动物。该疾病的防治是使用化学类杀真菌剂进行的，在某些情况下，化学类杀真菌剂已被观察到存在环境问题，例如抗污染性。一种替代方法是，利用我国植物区系的多样性，研究具有抗肝炎Fasciola肝功能的植物提取物。本文的目的是使用墨西哥民族植物学中使用的某些植物的抗寄生物提取物，鉴定，评估和阐明体外抗Fasciola肝的作用。我们收集，干燥，加工并在体外测试了约20株具有各自化学元素（己烷，乙酸乙酯和甲醇）的植物，获得了总共60种提取物的测试结果。进行了3天的体外评估，并将每种提取物的功效与未处理的对照组进行了比较。每种测试都用13种提取物重复6次，这些提取物显示出更大的fasciolocide活性。测试的这13种提取物的结果范围为80％至100％，所测试的植物为：Castela tortuosa（chaparro amargo），Achillea millefolium（plumajillo），百里香（thyme），Justicia spicigera（muicle），Climrida critridora（雪松），白杨（Alamo），薄荷（Mentha piperita），薄荷（Chenopodiumgravolens）（epazote de zorrillo），草木Lippiagravolens（oregano），墨西哥蒿（Artemisia mexicana）（雌蕊）和蒿蒿（Asemisia absinthium）（艾草）。容量;在所有试验中均使用500 gr / Lt的剂量。应该进行进一步的体外研究以获得每种提取物的LD50，以便能够分离出己烷变量中的主要活性成分。

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