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首页> 外文期刊>American Journal of Plant Sciences >Fast and Effective Thermotherapy Treatment for In Vitro Virus Eradication in Apple and Pear Trees
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Fast and Effective Thermotherapy Treatment for In Vitro Virus Eradication in Apple and Pear Trees

机译：快速有效的热疗法，用于消除苹果树和梨树中的体外病毒

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Heat therapy followed by the isolation and style="font-family:Verdana;">in vitro style="font-family:Verdana;"> culture of apical meristems is a suitable procedure for virus eradication. However, the period of heat treatment is usually long (28 style="font-family:;" "=""> style="font-family:Verdana;">- style="font-family:;" "=""> style="font-family:;" "=""> style="font-family:Verdana;">50 days) and the yield of viable plants free of viruses after treatment is often low (<50%). Here, we describe an alternative method to obtain virus-free plants. We used traditional Galician cultivars, six apple trees and two pear trees, infected with Apple chlorotic leaf spot virus (ACLSV) and Apple mosaic virus (ApMV). We combined heat therapy of style="font-family:Verdana;">in vitro style="font-family:Verdana;"> shoots using a temperature gradient from 25 style="white-space:nowrap;">℃ to 40 style="white-space:nowrap;">℃ increasing 1 style="white-space:nowrap;">℃ per day for a shorter period of time (18 days) with the posterior isolation and culture of apical meristems. All DNA samples analyzed, obtained from plants style="font-family:;" "=""> style="font-family:;" "=""> style="font-family:Verdana;">developed from meristems, were 100% free of ApMV and almost 90% free of ACLSV. With this style="font-family:Verdana;">in vitro style="font-family:Verdana;"> procedure combined we obtained a good yield of tested plants free of viruses. Our method is fast and effective and it could be also useful to eradicate these and other viruses in other fruit trees.
机译：热疗法，然后进行体外分离和 style =“ font-family：Verdana;”>体外 style =“ font-family：Verdana;”>根尖分生组织的培养是消灭病毒的合适方法。但是，热处理的时间通常较长（28 style =“ font-family :;”“ =”“> style =” font-family：Verdana;“>- style =“ font-family :;”“ =”“> style =” font-family :;“” =“”> style =“ font-family：Verdana ;“> 50天），处理后的无病毒活植物的产量通常很低（<50％）。在此，我们介绍了另一种获得无病毒植物的方法，我们使用了传统的加利西亚品种，六棵苹果树和两棵梨树，分别感染了苹果褪绿叶斑病毒（ACLSV）和苹果花叶病毒（ApMV）。我们结合 style =“ font-family：Verdana;”> / span> style =“ font-family：Verdana;”>使用从25 style =“ white-space：nowrap;”>℃到40 style的温度梯度进行拍摄=“ white-space：nowrap;”>℃每天增加1 style =“ white-space：nowrap;”>℃，在后部较短的时间段内（18天）顶缘分离和培养tems。从植物中获得的所有已分析的DNA样品 style =“ font-family :;” “ =”“> style =” font-family :;“” =“”> style =“ font-family：Verdana;”>从分生组织发展而来，它们100％不含ApMV，几乎90％无ACLSV。通过 style =“ font-family：Verdana;”>体外 style =“ font-family：Verdana;”>程序，我们获得了不含病毒的被测植物产量高。我们的方法快速有效，对于消除其他果树中的这些病毒和其他病毒也可能有用。

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