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首页> 外文期刊>Aging cell. >Plasticity of death rates in stationary phase in Saccharomyces?cerevisiae
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Plasticity of death rates in stationary phase in Saccharomyces?cerevisiae

机译：酿酒酵母固定期死亡率的可塑性

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For the species that have been most carefully studied, mortality rises with age and then plateaus or declines at advanced ages, except for yeast. Remarkably, mortality for yeast can rise, fall and rise again. In the present study we investigated (i) if this complicated shape could be modulated by environmental conditions by measuring mortality with different food media and temperature; (ii) if it is triggered by biological heterogeneity by measuring mortality in stationary phase in populations fractionated into subpopulations of young, virgin cells, and replicatively older, non-virgin cells. We also discussed the results of a staining method to measure viability instead of measuring the number of cells able to exit stationary phase and form a colony. We showed that different shapes of age-specific death rates were observed and that their appearance depended on the environmental conditions. Furthermore, biological heterogeneity explained the shapes of mortality with homogeneous populations of young, virgin cells exhibiting a simple shape of mortality in conditions under which more heterogeneous populations of older cells or unfractionated populations displayed complicated death rates. Finally, the staining method suggested that cells lost the capacity to exit stationary phase and to divide long before they died in stationary phase. These results explain a phenomenon that was puzzling because it appeared to reflect a radical departure from mortality patterns observed for other species.
机译：对于经过最仔细研究的物种，除酵母菌外，死亡率会随着年龄的增长而增加，然后达到平稳或下降。值得注意的是，酵母菌的死亡率可能会上升，下降并再次上升。在本研究中，我们研究（i）通过测量不同食物介质和温度下的死亡率，这种复杂形状是否可以被环境条件调节; （ii）是否由生物异质性触发，方法是通过测量分为原始年轻细胞和复制性较老非原始细胞亚群的种群固定期的死亡率来进行测量。我们还讨论了用于测量生存力的染色方法的结果，而不是测量能够离开固定相并形成菌落的细胞数量。我们发现观察到不同形状的特定年龄死亡率，并且它们的外观取决于环境条件。此外，生物学异质性解释了死亡率的形状，年轻的原始细胞的同质群体在较老细胞的更多异质群体或普通组分的群体显示出复杂的死亡率的情况下表现出简单的死亡率形状。最后，染色方法提示细胞丧失了离开固定相并分裂的能力，直到它们在固定相中死亡之前。这些结果解释了一个令人费解的现象，因为它似乎反映出与其他物种所观察到的死亡模式完全不同。

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